5K1X

Catalytic domain of polyspecific pyrrolysyl-tRNA synthetase mutant Y306A/N346A/C348A/Y384F in complex with AMPPNP

5K1X の概要

• エントリーDOI: 10.2210/pdb5k1x/pdb
• 関連するPDBエントリー: 5K1P
• 分子名称: Pyrrolysine--tRNA ligase, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, MAGNESIUM ION, ... (7 entities in total)
• 機能のキーワード: protein engineering, aminoacyl-trna synthetase, noncanonical amino acids, ligase
• 由来する生物種: Methanosarcina mazei
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32419.76

構造登録者

Weber, A. (登録日: 2016-05-18, 公開日: 2016-10-19, 最終更新日: 2024-03-06)

主引用文献

Lee, Y.J.,Schmidt, M.J.,Tharp, J.M.,Weber, A.,Koenig, A.L.,Zheng, H.,Gao, J.,Waters, M.L.,Summerer, D.,Liu, W.R.
Genetically encoded fluorophenylalanines enable insights into the recognition of lysine trimethylation by an epigenetic reader.
Chem.Commun.(Camb.), 52:12606-12609, 2016

PubMed Abstract: Fluorophenylalanines bearing 2-5 fluorine atoms at the phenyl ring have been genetically encoded by amber codon. Replacement of F59, a phenylalanine residue that is directly involved in interactions with trimethylated K9 of histone H3, in the Mpp8 chromodomain recombinantly with fluorophenylalanines significantly impairs the binding to a K9-trimethylated H3 peptide.

PubMed: 27711380
DOI: 10.1039/c6cc05959g

実験手法

X-RAY DIFFRACTION (1.95 Å)