5JVX

X-ray structure of the adduct formed in the reaction between thaumatin and a gold carbene compound

Summary for 5JVX

• Entry DOI: 10.2210/pdb5jvx/pdb
• Descriptor: Thaumatin-1, L(+)-TARTARIC ACID, (1-butyl-3-methyl-1,3-dihydro-2H-imidazol-2-ylidene)(chloro)gold, ... (5 entities in total)
• Functional Keywords: plant protein
• Biological source: Thaumatococcus daniellii (Katemfe)
• Cellular location: Cytoplasmic vesicle: P02883
• Total number of polymer chains: 1
• Total formula weight: 24786.47

Authors

Ferraro, G.,Merlino, A. (deposition date: 2016-05-11, release date: 2016-07-13, Last modification date: 2024-10-16)

Primary citation

Ferraro, G.,Gabbiani, C.,Merlino, A.
First Crystal Structure for a Gold Carbene-Protein Adduct.
Bioconjug.Chem., 27:1584-1587, 2016

PubMed Abstract: The X-ray structure of the adduct formed in the reaction between the gold N-heterocyclic carbene compound Au(NHC)Cl (with NHC = 1-butyl-3-methyl-imidazole-2-ylidene) and the model protein thaumatin is reported here. The structure reveals binding of Au(NHC)(+) fragments to distinct protein sites. Notably, binding of the gold compound occurs at lysine side chains and at the N-terminal tail; the metal binds the protein after releasing Cl(-) ligand, but retaining NHC fragment.

PubMed: 27364343
DOI: 10.1021/acs.bioconjchem.6b00298

Experimental method

X-RAY DIFFRACTION (1.7 Å)