5JVK
Structural insights into a family 39 glycoside hydrolase from the gut symbiont Bacteroides cellulosilyticus WH2.
Summary for 5JVK
| Entry DOI | 10.2210/pdb5jvk/pdb |
| Descriptor | Uncharacterized protein, SULFATE ION, GLYCEROL, ... (5 entities in total) |
| Functional Keywords | bacteroides, gh39, phylogeny, endo-glycoside hydrolase, hydrolase |
| Biological source | Bacteroides cellulosilyticus |
| Total number of polymer chains | 3 |
| Total formula weight | 196258.72 |
| Authors | Ali-Ahmad, A.,Garron, M.L.,Zamboni, V.,Lenfant, N.,Bourne, Y.,Vincent, F. (deposition date: 2016-05-11, release date: 2016-12-07, Last modification date: 2024-05-08) |
| Primary citation | Ali-Ahmad, A.,Garron, M.L.,Zamboni, V.,Lenfant, N.,Nurizzo, D.,Henrissat, B.,Berrin, J.G.,Bourne, Y.,Vincent, F. Structural insights into a family 39 glycoside hydrolase from the gut symbiont Bacteroides cellulosilyticus WH2. J. Struct. Biol., 197:227-235, 2017 Cited by PubMed Abstract: Bacteria from the human gut are equipped with an arsenal of carbohydrate-active enzymes that degrade dietary and host-derived glycans. In this study, we present the 2.5Å resolution crystal structure of a member (GH39wh2) from the human gut bacteria Bacteroides cellulosilyticus WH2 representative of a new subgroup within family GH39. Together with 6 other GHs, GH39wh2 belongs to a polysaccharide utilization locus (PUL) that could be involved in detecting, binding and hydrolysing a specific carbohydrate species from the intestinal tract. GH39wh2 shares a similar architecture as other members of family GH39 dominated by a typical (β/α)-barrel fold harboring the catalytic residues and decorated by β-sandwich accessory domains. The GH39wh2 structure unveils an atypical shallow groove rather than a deep pocket due to drastic rearrangements in surface loops surrounding the catalytic interface. These structural adaptations seem to favour recognition of large branched substrates and may explain the lack of activity of GH39wh2 toward small xylose-based and other typical substrates from GH39 members, emphasizing the molecular diversity within the GH39 family. A phylogenetic analysis of the entire GH39 family assigns GH39wh2 as a new subgroup, consistent with the extensive remodelling of the active site region that may confer new substrate specificity toward a complex glycan chain. PubMed: 27890857DOI: 10.1016/j.jsb.2016.11.004 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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