Summary for 5JVA
| Entry DOI | 10.2210/pdb5jva/pdb |
| Related | 3T6H |
| Descriptor | TagRFP-T, BETA-MERCAPTOETHANOL, SULFATE ION, ... (4 entities in total) |
| Functional Keywords | red fluorescent proteins, tagrfp-t, beta barrel, photostable, fluorescent protein |
| Biological source | synthetic construct |
| Total number of polymer chains | 4 |
| Total formula weight | 109116.31 |
| Authors | Hu, X.J. (deposition date: 2016-05-11, release date: 2017-05-17, Last modification date: 2024-04-03) |
| Primary citation | Liu, R.,Liang, Q.N.,Du, S.Q.,Hu, X.J.,Ding, Y. The crystal structure of red fluorescent protein TagRFP-T reveals the mechanism of its superior photostability. Biochem. Biophys. Res. Commun., 477:229-234, 2016 Cited by PubMed Abstract: The red fluorescent protein variant TagRFP-T has greatly improved photostability over its parent molecule, TagRFP, but the underlying mechanism leading to this improvement is to date unknown. The 1.95 Å resolution crystallographic structure of TagRFP-T showed that its chromophore exists as a mixture of cis and trans coplanar isomers in roughly equal proportions. Interestingly, both isomers are able to fluoresce, a property that has never been observed in any other fluorescent protein. We propose a "circular restoration model" for TagRFP-T to explain its superior photostability: There are four co-existing chromophore states (cis/trans protonated/ionized state) that can be driven by light to transform from one state into another. This model also explains how TagRPF-T essentially eliminates the temporary dark state (reversible photobleaching). PubMed: 27297107DOI: 10.1016/j.bbrc.2016.06.047 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.95 Å) |
Structure validation
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