5JT8

Structural basis for the limited antibody cross reactivity between the mite allergens Blo t 1 and Der p 1

5JT8 の概要

• エントリーDOI: 10.2210/pdb5jt8/pdb
• 分子名称: Blo t 1 allergen, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: allergen mite, allergen
• 由来する生物種: Blomia tropicalis (Mite)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 77604.36

構造登録者

Meno, K.,Kastrup, J.S.,Gajhede, M. (登録日: 2016-05-09, 公開日: 2017-05-24, 最終更新日: 2024-10-23)

主引用文献

Meno, K.H.,Kastrup, J.S.,Kuo, I.C.,Chua, K.Y.,Gajhede, M.
The structure of the mite allergen Blo t 1 explains the limited antibody cross-reactivity to Der p 1.
Allergy, 72:665-670, 2017

PubMed Abstract: The Blomia tropicalis (Blo t) mite species is considered a storage mite in temperate climate zones and an important source of indoor allergens causing allergic asthma and rhinitis in tropical and subtropical regions. Here, we report the crystal structure of one of the allergens from Blo t, recombinant proBlo t 1 (rproBlo t 1), determined at 2.1 Å resolution. Overall, the fold of rproBlo t 1 is characteristic for the pro-form of cysteine proteases from the C1A class. Structural comparison of experimentally mapped Der f 1/Der p1 IgG epitopes to the same surface patch on Blo t 1, as well as of sequence identity of surface-exposed residues, suggests limited cross-reactivity between these allergens and Blo t 1. This is in agreement with ELISA inhibition results showing that, although cross-reactive human IgE epitopes exist, there are unique IgE epitopes for both Blo t 1 and Der p 1.

PubMed: 27997997
DOI: 10.1111/all.13111

実験手法

X-RAY DIFFRACTION (2.1 Å)