5JOE

Crystal structure of I81 from titin

Summary for 5JOE

• Entry DOI: 10.2210/pdb5joe/pdb
• Descriptor: Titin, ISOPROPYL ALCOHOL, 1,2-ETHANEDIOL, ... (4 entities in total)
• Functional Keywords: titin, immunoglobulin, structural protein, transferase
• Biological source: Homo sapiens (Human)
• Cellular location: Cytoplasm : Q8WZ42
• Total number of polymer chains: 1
• Total formula weight: 10639.22

Authors

Fleming, J.,Zhou, T.,Bogomolovas, J.,Labeit, S.,Mayans, O. (deposition date: 2016-05-02, release date: 2016-08-17, Last modification date: 2024-01-10)

Primary citation

Zhou, T.,Fleming, J.R.,Franke, B.,Bogomolovas, J.,Barsukov, I.,Rigden, D.J.,Labeit, S.,Mayans, O.
CARP interacts with titin at a unique helical N2A sequence and at the domain Ig81 to form a structured complex.
Febs Lett., 590:3098-3110, 2016

PubMed Abstract: The cardiac ankyrin repeat protein (CARP) is up-regulated in the myocardium during cardiovascular disease and in response to mechanical or toxic stress. Stress-induced CARP interacts with the N2A spring region of the titin filament to modulate muscle compliance. We characterize the interaction between CARP and titin-N2A and show that the binding site in titin spans the dual domain UN2A-Ig81. We find that the unique sequence UN2A is not structurally disordered, but that it has a stable, elongated α-helical fold that possibly acts as a constant force spring. Our findings portray CARP/titin-N2A as a structured node and help to rationalize the molecular basis of CARP mechanosensing in the sarcomeric I-band.

PubMed: 27531639
DOI: 10.1002/1873-3468.12362

Experimental method

X-RAY DIFFRACTION (2 Å)