5JKL

Binary crystal structure of positively and negatively supercharged variants Ftn(pos) and Ftn(neg) from human heavy chain ferritin (Mg formate condition)

5JKL の概要

• エントリーDOI: 10.2210/pdb5jkl/pdb
• 関連するPDBエントリー: 5JKM
• 分子名称: Ferritin heavy chain, FE (III) ION, MAGNESIUM ION, ... (7 entities in total)
• 機能のキーワード: protein design, protein engineering, charged protein containers, binary protein structures, self-assembly, binary nanoparticle superlattices, oxidoreductase
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 258963.25

構造登録者

Kuenzle, M.,Beck, T. (登録日: 2016-04-26, 公開日: 2016-10-26, 最終更新日: 2024-01-10)

主引用文献

Kunzle, M.,Eckert, T.,Beck, T.
Binary Protein Crystals for the Assembly of Inorganic Nanoparticle Superlattices.
J.Am.Chem.Soc., 138:12731-12734, 2016

PubMed Abstract: Biomolecules can act as functional templates for the organization of inorganic particles. Here we use two protein containers, engineered with opposite surface charge, as building blocks for the construction of a new type of biohybrid material. Binary structures with crystalline order were obtained, adopting a tetragonal lattice. Moreover, the cavity of the engineered protein containers can be filled with inorganic nanoparticles. The controlled assembly of these protein-nanoparticle composites yields highly ordered binary nanoparticle superlattices as free-standing crystals, with up to a few hundred micrometers in size. Because the structure and lattice parameters of the protein-nanoparticle crystals are independent of their nanoparticle cargo, the binary protein material may serve as a generally applicable matrix for the assembly of a variety of nanoparticles types.

PubMed: 27617514
DOI: 10.1021/jacs.6b07260

実験手法

X-RAY DIFFRACTION (1.8 Å)