5JJI

Rho transcription termination factor bound to rU7 and 6 ADP-BeF3 molecules

5JJI の概要

• エントリーDOI: 10.2210/pdb5jji/pdb
• 関連するPDBエントリー: 5JJK 5JJL
• 分子名称: Transcription termination factor Rho, rU12: 5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)- 3', ADENOSINE-5'-DIPHOSPHATE, ... (7 entities in total)
• 機能のキーワード: protein-rna complex, transcription-rna complex, transcription/rna
• 由来する生物種: Escherichia coli O157:H7; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 294549.28

構造登録者

Thomsen, N.D.,Lawson, M.R.,Witkowsky, L.B.,Qu, S.,Berger, J.M. (登録日: 2016-04-24, 公開日: 2016-11-16, 最終更新日: 2024-10-09)

主引用文献

Thomsen, N.D.,Lawson, M.R.,Witkowsky, L.B.,Qu, S.,Berger, J.M.
Molecular mechanisms of substrate-controlled ring dynamics and substepping in a nucleic acid-dependent hexameric motor.
Proc. Natl. Acad. Sci. U.S.A., 113:E7691-E7700, 2016

PubMed Abstract: Ring-shaped hexameric helicases and translocases support essential DNA-, RNA-, and protein-dependent transactions in all cells and many viruses. How such systems coordinate ATPase activity between multiple subunits to power conformational changes that drive the engagement and movement of client substrates is a fundamental question. Using the Escherichia coli Rho transcription termination factor as a model system, we have used solution and crystallographic structural methods to delineate the range of conformational changes that accompany distinct substrate and nucleotide cofactor binding events. Small-angle X-ray scattering data show that Rho preferentially adopts an open-ring state in solution and that RNA and ATP are both required to cooperatively promote ring closure. Multiple closed-ring structures with different RNA substrates and nucleotide occupancies capture distinct catalytic intermediates accessed during translocation. Our data reveal how RNA-induced ring closure templates a sequential ATP-hydrolysis mechanism, provide a molecular rationale for how the Rho ATPase domains distinguishes between distinct RNA sequences, and establish structural snapshots of substepping events in a hexameric helicase/translocase.

PubMed: 27856760
DOI: 10.1073/pnas.1616745113

実験手法

X-RAY DIFFRACTION (2.601 Å)