5JH5

Structural Basis for the Hierarchical Assembly of the Core of PRC1.1

5JH5 の概要

• エントリーDOI: 10.2210/pdb5jh5/pdb
• 分子名称: Lysine-specific demethylase 2B, S-phase kinase-associated protein 1, Polycomb group RING finger protein 1, ... (5 entities in total)
• 機能のキーワード: gene repression, complex, transcription regulation, transcription repressor, metal binding protein-transcription complex, metal binding protein/transcription
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus, nucleolus : Q8NHM5; Nucleus : Q9BSM1 Q5H9F3
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 78554.55

構造登録者

Wong, S.J.,Taylor, A.B.,Hart, P.J.,Kim, C.A. (登録日: 2016-04-20, 公開日: 2016-09-14, 最終更新日: 2024-10-23)

主引用文献

Wong, S.J.,Gearhart, M.D.,Taylor, A.B.,Nanyes, D.R.,Ha, D.J.,Robinson, A.K.,Artigas, J.A.,Lee, O.J.,Demeler, B.,Hart, P.J.,Bardwell, V.J.,Kim, C.A.
KDM2B Recruitment of the Polycomb Group Complex, PRC1.1, Requires Cooperation between PCGF1 and BCORL1.
Structure, 24:1795-1801, 2016

PubMed Abstract: KDM2B recruits H2A-ubiquitinating activity of a non-canonical Polycomb Repression Complex 1 (PRC1.1) to CpG islands, facilitating gene repression. We investigated the molecular basis of recruitment using in vitro assembly assays to identify minimal components, subcomplexes, and domains required for recruitment. A minimal four-component PRC1.1 complex can be assembled by combining two separately isolated subcomplexes: the DNA-binding KDM2B/SKP1 heterodimer and the heterodimer of BCORL1 and PCGF1, a core component of PRC1.1. The crystal structure of the KDM2B/SKP1/BCORL1/PCGF1 complex illustrates the crucial role played by the PCGF1/BCORL1 heterodimer. The BCORL1 PUFD domain positions residues preceding the RAWUL domain of PCGF1 to create an extended interface for interaction with KDM2B, which is unique to the PCGF1-containing PRC1.1 complex. The structure also suggests how KDM2B might simultaneously function in PRC1.1 and an SCF ubiquitin ligase complex and the possible molecular consequences of BCOR PUFD internal tandem duplications found in pediatric kidney and brain tumors.

PubMed: 27568929
DOI: 10.1016/j.str.2016.07.011

実験手法

X-RAY DIFFRACTION (2.55 Å)