5JGP
Crystal structure of the nitrate/nitrite sensor NarQ fragment bound with iodide ions
Summary for 5JGP
| Entry DOI | 10.2210/pdb5jgp/pdb |
| Related | 5IJI |
| Descriptor | Nitrate/nitrite sensor protein NarQ, NITRATE ION, IODIDE ION, ... (4 entities in total) |
| Functional Keywords | membrane protein, sensor, histidine kinase, iodide, transferase, signaling protein |
| Biological source | Escherichia coli (strain K12) |
| Cellular location | Cell inner membrane ; Multi-pass membrane protein : P27896 |
| Total number of polymer chains | 1 |
| Total formula weight | 28397.75 |
| Authors | Melnikov, I.,Polovinkin, V.,Popov, A.,Gordeliy, V. (deposition date: 2016-04-20, release date: 2017-05-31, Last modification date: 2025-10-01) |
| Primary citation | Melnikov, I.,Polovinkin, V.,Kovalev, K.,Gushchin, I.,Shevtsov, M.,Shevchenko, V.,Mishin, A.,Alekseev, A.,Rodriguez-Valera, F.,Borshchevskiy, V.,Cherezov, V.,Leonard, G.A.,Gordeliy, V.,Popov, A. Fast iodide-SAD phasing for high-throughput membrane protein structure determination. Sci Adv, 3:e1602952-e1602952, 2017 Cited by PubMed Abstract: We describe a fast, easy, and potentially universal method for the de novo solution of the crystal structures of membrane proteins via iodide-single-wavelength anomalous diffraction (I-SAD). The potential universality of the method is based on a common feature of membrane proteins-the availability at the hydrophobic-hydrophilic interface of positively charged amino acid residues with which iodide strongly interacts. We demonstrate the solution using I-SAD of four crystal structures representing different classes of membrane proteins, including a human G protein-coupled receptor (GPCR), and we show that I-SAD can be applied using data collection strategies based on either standard or serial x-ray crystallography techniques. PubMed: 28508075DOI: 10.1126/sciadv.1602952 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.7 Å) |
Structure validation
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