5JCA

NADP(H) bound NADH-dependent Ferredoxin:NADP Oxidoreductase (NfnI) from Pyrococcus furiosus

Summary for 5JCA

• Entry DOI: 10.2210/pdb5jca/pdb
• Descriptor: NADH-dependent Ferredoxin:NADP Oxidoreductase (NfnI) subunit alpha, NADH-dependent Ferredoxin:NADP Oxidoreductase (NfnI) subunit beta, IRON/SULFUR CLUSTER, ... (9 entities in total)
• Functional Keywords: nfni, oxidoreductase, pyrococcus furiosus, nadp(h) bound nfni
• Biological source: Pyrococcus furiosus; More
• Cellular location: Cytoplasm : Q8U195 Q8U194
• Total number of polymer chains: 2
• Total formula weight: 87665.93

Authors

Zadvornyy, O.A.,Schut, G.J.,Nguyen, D.M.,Artz, J.H.,Tokmina-Lukaszewska, M.,Lipscomb, G.,Adams, M.W.,Peters, J.W. (deposition date: 2016-04-14, release date: 2017-04-12, Last modification date: 2024-05-22)

Primary citation

Lubner, C.E.,Jennings, D.P.,Mulder, D.W.,Schut, G.J.,Zadvornyy, O.A.,Hoben, J.P.,Tokmina-Lukaszewska, M.,Berry, L.,Nguyen, D.M.,Lipscomb, G.L.,Bothner, B.,Jones, A.K.,Miller, A.F.,King, P.W.,Adams, M.W.W.,Peters, J.W.
Mechanistic insights into energy conservation by flavin-based electron bifurcation.
Nat. Chem. Biol., 13:655-659, 2017

PubMed Abstract: The recently realized biochemical phenomenon of energy conservation through electron bifurcation provides biology with an elegant means to maximize utilization of metabolic energy. The mechanism of coordinated coupling of exergonic and endergonic oxidation-reduction reactions by a single enzyme complex has been elucidated through optical and paramagnetic spectroscopic studies revealing unprecedented features. Pairs of electrons are bifurcated over more than 1 volt of electrochemical potential by generating a low-potential, highly energetic, unstable flavin semiquinone and directing electron flow to an iron-sulfur cluster with a highly negative potential to overcome the barrier of the endergonic half reaction. The unprecedented range of thermodynamic driving force that is generated by flavin-based electron bifurcation accounts for unique chemical reactions that are catalyzed by these enzymes.

PubMed: 28394885
DOI: 10.1038/nchembio.2348

Experimental method

X-RAY DIFFRACTION (1.5 Å)