5J91
Structure of the Wild-type 70S E coli ribosome bound to Tigecycline
This is a non-PDB format compatible entry.
Summary for 5J91
| Entry DOI | 10.2210/pdb5j91/pdb |
| Related | 5IT8 5J5B 5J7L 5J88 5J8A 5JC9 |
| Descriptor | 16S rRNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (70 entities in total) |
| Functional Keywords | tigecycline, ribosome, escherichia coli, ribosome-inhibitor complex, ribosome-antibiotic complex, ribosome/antibiotic |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 105 |
| Total formula weight | 4272877.52 |
| Authors | Cocozaki, A.,Ferguson, A. (deposition date: 2016-04-08, release date: 2016-07-06, Last modification date: 2025-03-19) |
| Primary citation | Cocozaki, A.I.,Altman, R.B.,Huang, J.,Buurman, E.T.,Kazmirski, S.L.,Doig, P.,Prince, D.B.,Blanchard, S.C.,Cate, J.H.,Ferguson, A.D. Resistance mutations generate divergent antibiotic susceptibility profiles against translation inhibitors. Proc.Natl.Acad.Sci.USA, 113:8188-8193, 2016 Cited by PubMed Abstract: Mutations conferring resistance to translation inhibitors often alter the structure of rRNA. Reduced susceptibility to distinct structural antibiotic classes may, therefore, emerge when a common ribosomal binding site is perturbed, which significantly reduces the clinical utility of these agents. The translation inhibitors negamycin and tetracycline interfere with tRNA binding to the aminoacyl-tRNA site on the small 30S ribosomal subunit. However, two negamycin resistance mutations display unexpected differential antibiotic susceptibility profiles. Mutant U1060A in 16S Escherichia coli rRNA is resistant to both antibiotics, whereas mutant U1052G is simultaneously resistant to negamycin and hypersusceptible to tetracycline. Using a combination of microbiological, biochemical, single-molecule fluorescence transfer experiments, and X-ray crystallography, we define the specific structural defects in the U1052G mutant 70S E. coli ribosome that explain its divergent negamycin and tetracycline susceptibility profiles. Unexpectedly, the U1052G mutant ribosome possesses a second tetracycline binding site that correlates with its hypersusceptibility. The creation of a previously unidentified antibiotic binding site raises the prospect of identifying similar phenomena in antibiotic-resistant pathogens in the future. PubMed: 27382179DOI: 10.1073/pnas.1605127113 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.96 Å) |
Structure validation
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