5J6X

Crystal structure of the apo-Zalpha of Zebrafish PKZ

5J6X の概要

• エントリーDOI: 10.2210/pdb5j6x/pdb
• 分子名称: Z-DNA binding protein kinase (2 entities in total)
• 機能のキーワード: pkz zdna zalpha whth, dna binding protein
• 由来する生物種: Danio rerio (Zebrafish)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 17075.58

構造登録者

Subramani, V.K.,Kim, D.,Kim, K.K. (登録日: 2016-04-05, 公開日: 2016-09-07, 最終更新日: 2023-11-08)

主引用文献

Subramani, V.K.,Kim, D.,Yun, K.,Kim, K.K.
Structural and functional studies of a large winged Z-DNA-binding domain of Danio rerio protein kinase PKZ
Febs Lett., 590:2275-2285, 2016

PubMed Abstract: The Z-DNA-binding domain of PKZ from zebrafish (Danio rerio; drZαPKZ ) contains the largest β-wing among known Z-DNA-binding domains. To elucidate the functional implication of the β-wing, we solved the crystal structure of apo-drZαPKZ . Structural comparison with its Z-DNA-bound form revealed a large conformational change within the β-wing during Z-DNA binding. Biochemical studies of protein mutants revealed that two basic residues in the β-wing are responsible for Z-DNA recognition as well as fast B-Z transition. Therefore, the extra basic residues in the β-wing of drZαPKZ are necessary for the fast B-Z transition activity.

PubMed: 27265117
DOI: 10.1002/1873-3468.12238

実験手法

X-RAY DIFFRACTION (2.493 Å)