5J4Z

Architecture of tight respirasome

これはPDB形式変換不可エントリーです。

5J4Z の概要

• エントリーDOI: 10.2210/pdb5j4z/pdb
• EMDBエントリー: 8130
• 分子名称: COMPLEX I ND3, COMPLEX I ND6, COMPLEX I ND4L, ... (80 entities in total)
• 機能のキーワード: membrane protein complex, supercomplex, respirasome, electron transport
• 由来する生物種: Ovis aries (SHEEP); 詳細
• タンパク質・核酸の鎖数: 89
• 化学式量合計: 1285297.38

構造登録者

Letts, J.A.,Fiedorczuk, K.,Sazanov, L.A. (登録日: 2016-04-01, 公開日: 2016-09-21, 最終更新日: 2024-10-23)

主引用文献

Letts, J.A.,Fiedorczuk, K.,Sazanov, L.A.
The architecture of respiratory supercomplexes.
Nature, 537:644-648, 2016

PubMed Abstract: Mitochondrial electron transport chain complexes are organized into supercomplexes responsible for carrying out cellular respiration. Here we present three architectures of mammalian (ovine) supercomplexes determined by cryo-electron microscopy. We identify two distinct arrangements of supercomplex CICIIICIV (the respirasome)-a major 'tight' form and a minor 'loose' form (resolved at the resolution of 5.8 Å and 6.7 Å, respectively), which may represent different stages in supercomplex assembly or disassembly. We have also determined an architecture of supercomplex CICIII at 7.8 Å resolution. All observed density can be attributed to the known 80 subunits of the individual complexes, including 132 transmembrane helices. The individual complexes form tight interactions that vary between the architectures, with complex IV subunit COX7a switching contact from complex III to complex I. The arrangement of active sites within the supercomplex may help control reactive oxygen species production. To our knowledge, these are the first complete architectures of the dominant, physiologically relevant state of the electron transport chain.

PubMed: 27654913
DOI: 10.1038/nature19774

実験手法

ELECTRON MICROSCOPY (5.8 Å)