5J01

Structure of the lariat form of a chimeric derivative of the Oceanobacillus iheyensis group II intron in the presence of NH4+ and MG2+.

5J01 の概要

• エントリーDOI: 10.2210/pdb5j01/pdb
• 分子名称: group II intron lariat, MAGNESIUM ION, AMMONIUM ION, ... (4 entities in total)
• 機能のキーワード: group ii intron, lariat, 2'-5' phosphodiester bond, ribozyme, rna catalysis, self-splicing, rna
• 由来する生物種: Oceanobacillus iheyensis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 136698.30

構造登録者

Costa, M.,Walbott, H.,Monachello, D.,Westhof, E.,Michel, F. (登録日: 2016-03-26, 公開日: 2016-12-14, 最終更新日: 2024-01-10)

主引用文献

Costa, M.,Walbott, H.,Monachello, D.,Westhof, E.,Michel, F.
Crystal structures of a group II intron lariat primed for reverse splicing.
Science, 354:-, 2016

PubMed Abstract: The 2'-5' branch of nuclear premessenger introns is believed to have been inherited from self-splicing group II introns, which are retrotransposons of bacterial origin. Our crystal structures at 3.4 and 3.5 angstrom of an excised group II intron in branched ("lariat") form show that the 2'-5' branch organizes a network of active-site tertiary interactions that position the intron terminal 3'-hydroxyl group into a configuration poised to initiate reverse splicing, the first step in retrotransposition. Moreover, the branchpoint and flanking helices must undergo a base-pairing switch after branch formation. A group II-based model of the active site of the nuclear splicing machinery (the spliceosome) is proposed. The crucial role of the lariat conformation in active-site assembly and catalysis explains its prevalence in modern splicing.

PubMed: 27934709
DOI: 10.1126/science.aaf9258

実験手法

X-RAY DIFFRACTION (3.39 Å)