5IUV
Crystal Structure of Indole-3-acetaldehyde Dehydrogenase in complexed with NAD+
Summary for 5IUV
| Entry DOI | 10.2210/pdb5iuv/pdb |
| Related | 5IUU 5IUW |
| Descriptor | Aldehyde dehydrogenase family protein, NICOTINAMIDE-ADENINE-DINUCLEOTIDE (3 entities in total) |
| Functional Keywords | indole-3-acetaldehyde dehydrogenase, aldehyde dehydrogenase, oxidoreductase |
| Biological source | Pseudomonas syringae pv. tomato (strain DC3000) |
| Total number of polymer chains | 2 |
| Total formula weight | 106886.50 |
| Authors | Lee, S.G.,McClerklin, S.,Kunkel, B.,Jez, J.M. (deposition date: 2016-03-18, release date: 2017-10-25, Last modification date: 2023-09-27) |
| Primary citation | McClerklin, S.A.,Lee, S.G.,Harper, C.P.,Nwumeh, R.,Jez, J.M.,Kunkel, B.N. Indole-3-acetaldehyde dehydrogenase-dependent auxin synthesis contributes to virulence of Pseudomonas syringae strain DC3000. PLoS Pathog., 14:e1006811-e1006811, 2018 Cited by PubMed Abstract: The bacterial pathogen Pseudomonas syringae modulates plant hormone signaling to promote infection and disease development. P. syringae uses several strategies to manipulate auxin physiology in Arabidopsis thaliana to promote pathogenesis, including its synthesis of indole-3-acetic acid (IAA), the predominant form of auxin in plants, and production of virulence factors that alter auxin responses in the host; however, the role of pathogen-derived auxin in P. syringae pathogenesis is not well understood. Here we demonstrate that P. syringae strain DC3000 produces IAA via a previously uncharacterized pathway and identify a novel indole-3-acetaldehyde dehydrogenase, AldA, that functions in IAA biosynthesis by catalyzing the NAD-dependent formation of IAA from indole-3-acetaldehyde (IAAld). Biochemical analysis and solving of the 1.9 Å resolution x-ray crystal structure reveal key features of AldA for IAA synthesis, including the molecular basis of substrate specificity. Disruption of aldA and a close homolog, aldB, lead to reduced IAA production in culture and reduced virulence on A. thaliana. We use these mutants to explore the mechanism by which pathogen-derived auxin contributes to virulence and show that IAA produced by DC3000 suppresses salicylic acid-mediated defenses in A. thaliana. Thus, auxin is a DC3000 virulence factor that promotes pathogenicity by suppressing host defenses. PubMed: 29293681DOI: 10.1371/journal.ppat.1006811 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.928 Å) |
Structure validation
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