5IU1

N-terminal PAS domain homodimer of PpANR MAP3K from Physcomitrella patens.

Summary for 5IU1

• Entry DOI: 10.2210/pdb5iu1/pdb
• Descriptor: CTR1-like protein, GLYCEROL, SULFATE ION, ... (5 entities in total)
• Functional Keywords: homodimer, map3k, plant protein
• Biological source: Physcomitrella patens subsp. patens (Moss)
• Total number of polymer chains: 2
• Total formula weight: 26399.82

Authors

Stevenson, R.R.,Trinh, C.H.,Edwards, T.A.,Cuming, A.C. (deposition date: 2016-03-17, release date: 2016-06-01, Last modification date: 2024-10-23)

Primary citation

Stevenson, S.R.,Kamisugi, Y.,Trinh, C.H.,Schmutz, J.,Jenkins, J.W.,Grimwood, J.,Muchero, W.,Tuskan, G.A.,Rensing, S.A.,Lang, D.,Reski, R.,Melkonian, M.,Rothfels, C.J.,Li, F.W.,Larsson, A.,Wong, G.K.,Edwards, T.A.,Cuming, A.C.
Genetic Analysis of Physcomitrella patens Identifies ABSCISIC ACID NON-RESPONSIVE, a Regulator of ABA Responses Unique to Basal Land Plants and Required for Desiccation Tolerance.
Plant Cell, 28:1310-1327, 2016

PubMed Abstract: The anatomically simple plants that first colonized land must have acquired molecular and biochemical adaptations to drought stress. Abscisic acid (ABA) coordinates responses leading to desiccation tolerance in all land plants. We identified ABA nonresponsive mutants in the model bryophyte Physcomitrella patens and genotyped a segregating population to map and identify the ABA NON-RESPONSIVE (ANR) gene encoding a modular protein kinase comprising an N-terminal PAS domain, a central EDR domain, and a C-terminal MAPKKK-like domain. anr mutants fail to accumulate dehydration tolerance-associated gene products in response to drought, ABA, or osmotic stress and do not acquire ABA-dependent desiccation tolerance. The crystal structure of the PAS domain, determined to 1.7-Å resolution, shows a conserved PAS-fold that dimerizes through a weak dimerization interface. Targeted mutagenesis of a conserved tryptophan residue within the PAS domain generates plants with ABA nonresponsive growth and strongly attenuated ABA-responsive gene expression, whereas deleting this domain retains a fully ABA-responsive phenotype. ANR orthologs are found in early-diverging land plant lineages and aquatic algae but are absent from more recently diverged vascular plants. We propose that ANR genes represent an ancestral adaptation that enabled drought stress survival of the first terrestrial colonizers but were lost during land plant evolution.

PubMed: 27194706
DOI: 10.1105/tpc.16.00091

Experimental method

X-RAY DIFFRACTION (1.7 Å)