5ITZ

Crystal structure of the SAC domain of CPAP in a complex with Tubulin and Darpin

5ITZ の概要

• エントリーDOI: 10.2210/pdb5itz/pdb
• 分子名称: Tubulin alpha-1B chain, Tubulin beta-2B chain, Centromere protein J, ... (9 entities in total)
• 機能のキーワード: tubulin, cpap, centriole, structural protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 129839.54

構造登録者

Sharma, A.,Steinmetz, M.O. (登録日: 2016-03-17, 公開日: 2016-06-01, 最終更新日: 2024-01-10)

主引用文献

Sharma, A.,Aher, A.,Dynes, N.J.,Frey, D.,Katrukha, E.A.,Jaussi, R.,Grigoriev, I.,Croisier, M.,Kammerer, R.A.,Akhmanova, A.,Gonczy, P.,Steinmetz, M.O.
Centriolar CPAP/SAS-4 Imparts Slow Processive Microtubule Growth.
Dev.Cell, 37:362-376, 2016

PubMed Abstract: Centrioles are fundamental and evolutionarily conserved microtubule-based organelles whose assembly is characterized by microtubule growth rates that are orders of magnitude slower than those of cytoplasmic microtubules. Several centriolar proteins can interact with tubulin or microtubules, but how they ensure the exceptionally slow growth of centriolar microtubules has remained mysterious. Here, we bring together crystallographic, biophysical, and reconstitution assays to demonstrate that the human centriolar protein CPAP (SAS-4 in worms and flies) binds and "caps" microtubule plus ends by associating with a site of β-tubulin engaged in longitudinal tubulin-tubulin interactions. Strikingly, we uncover that CPAP activity dampens microtubule growth and stabilizes microtubules by inhibiting catastrophes and promoting rescues. We further establish that the capping function of CPAP is important to limit growth of centriolar microtubules in cells. Our results suggest that CPAP acts as a molecular lid that ensures slow assembly of centriolar microtubules and, thereby, contributes to organelle length control.

PubMed: 27219064
DOI: 10.1016/j.devcel.2016.04.024

実験手法

X-RAY DIFFRACTION (2.2 Å)