5ION

Solution Structure of Miz-1 Zinc Finger 13

Summary for 5ION

• Entry DOI: 10.2210/pdb5ion/pdb
• NMR Information: BMRB: 30032
• Descriptor: Zinc finger and BTB domain-containing protein 17, ZINC ION (2 entities in total)
• Functional Keywords: c2h2 zinc finger, miz-1, zbtb17, transcription factor, transcription
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 3298.04

Authors

Tremblay, C.,Bedard, M.,Lavigne, P. (deposition date: 2016-03-08, release date: 2016-03-30, Last modification date: 2024-05-15)

Primary citation

Tremblay, C.,Bedard, M.,Bonin, M.A.,Lavigne, P.
Solution structure of the 13th C2H2 Zinc Finger of Miz-1.
Biochem.Biophys.Res.Commun., 473:471-475, 2016

PubMed Abstract: Miz-1 is a BTB/POZ transcription factor that contains 13C2H2 Zinc Finger domains (ZF). Miz-1 transactivates and represses the transcription of a myriad of genes involved in many aspects of the biology of the cell. The detailed molecular interactions through which Miz-1 controls transcription, including its specific DNA binding via its ZF domains, remain to be understood and documented. In our effort to shed light into the structural biology of Miz-1, we have undertaken the determination of the structure of all its ZF and the characterization of their interactions with cognate DNA. The structure of ZF 1 to 10 have already been solved and characterized. Here, we present the structure of the synthetic Miz-1 ZF13 determined by 2D (1)H-(1)H NMR.

PubMed: 26972249
DOI: 10.1016/j.bbrc.2016.03.034

Experimental method

SOLUTION NMR