5IM4

Crystal structure of designed two-component self-assembling icosahedral cage I52-32

5IM4 の概要

• エントリーDOI: 10.2210/pdb5im4/pdb
• 分子名称: 6,7-dimethyl-8-ribityllumazine synthase, Phosphotransferase system, mannose/fructose-specific component IIA (2 entities in total)
• 機能のキーワード: icosahedron, designed protein cage, two-component, computational design, protein engineering, rosetta, self-assembling, co-assembling, multimerization, symmetry, nanomaterial, nanostructure, protein binding
• 由来する生物種: Candida albicans P37005; 詳細
• タンパク質・核酸の鎖数: 40
• 化学式量合計: 665301.76

構造登録者

Liu, Y.A.,Cascio, D.,Sawaya, M.R.,Bale, J.B.,Collazo, M.J.,Thomas, C.,Sheffler, W.,King, N.P.,Baker, D.,Yeates, T.O. (登録日: 2016-03-05, 公開日: 2016-07-27, 最終更新日: 2023-09-27)

主引用文献

Bale, J.B.,Gonen, S.,Liu, Y.,Sheffler, W.,Ellis, D.,Thomas, C.,Cascio, D.,Yeates, T.O.,Gonen, T.,King, N.P.,Baker, D.
Accurate design of megadalton-scale two-component icosahedral protein complexes.
Science, 353:389-394, 2016

PubMed Abstract: Nature provides many examples of self- and co-assembling protein-based molecular machines, including icosahedral protein cages that serve as scaffolds, enzymes, and compartments for essential biochemical reactions and icosahedral virus capsids, which encapsidate and protect viral genomes and mediate entry into host cells. Inspired by these natural materials, we report the computational design and experimental characterization of co-assembling, two-component, 120-subunit icosahedral protein nanostructures with molecular weights (1.8 to 2.8 megadaltons) and dimensions (24 to 40 nanometers in diameter) comparable to those of small viral capsids. Electron microscopy, small-angle x-ray scattering, and x-ray crystallography show that 10 designs spanning three distinct icosahedral architectures form materials closely matching the design models. In vitro assembly of icosahedral complexes from independently purified components occurs rapidly, at rates comparable to those of viral capsids, and enables controlled packaging of molecular cargo through charge complementarity. The ability to design megadalton-scale materials with atomic-level accuracy and controllable assembly opens the door to a new generation of genetically programmable protein-based molecular machines.

PubMed: 27463675
DOI: 10.1126/science.aaf8818

実験手法

X-RAY DIFFRACTION (3.5 Å)