5ILF

The X-ray structure of the adduct formed in the reaction between hen egg white lysozyme and compound 4, a platin(II) compound containing a O, S bidentate ligand

Summary for 5ILF

• Entry DOI: 10.2210/pdb5ilf/pdb
• Descriptor: Lysozyme C, DIMETHYL SULFOXIDE, PLATINUM (II) ION, ... (7 entities in total)
• Functional Keywords: metallodrug, protein platination, hydrolase
• Biological source: Gallus gallus (Chicken)
• Cellular location: Secreted: P00698
• Total number of polymer chains: 1
• Total formula weight: 14828.22

Authors

Merlino, A.,Ferraro, G. (deposition date: 2016-03-04, release date: 2016-12-07, Last modification date: 2024-11-13)

Primary citation

Hildebrandt, J.,Hafner, N.,Gorls, H.,Kritsch, D.,Ferraro, G.,Durst, M.,Runnebaum, I.B.,Merlino, A.,Weigand, W.
Platinum(ii) O,S complexes as potential metallodrugs against Cisplatin resistance.
Dalton Trans, 45:18876-18891, 2016

PubMed Abstract: We report on platinum(ii) complexes with different cinnamic acid derivatives as ligands with cytotoxic activity against Cisplatin resistant ovarian cancer cell line subcultures of SKOV3 and A2780. A typical mechanism of action for platinum(ii) complexes as Cisplatin itself is binding to the DNA and inducing double-strand breaks. We examined the biological behavior of these potential drugs with 9-methylguanine using NMR spectroscopic methods and their DNA damage potential including γH2AX-foci analyses. X-ray diffraction methods have been used to elucidate the molecular structures of the platinum(ii) complexes. Interactions with the model protein lysozyme have been evaluated by different techniques including UV-Vis absorption spectroscopy, fluorescence and X-ray crystallography.

PubMed: 27897281
DOI: 10.1039/c6dt01388k

Experimental method

X-RAY DIFFRACTION (1.85 Å)