5I9S
MicroED structure of proteinase K at 1.75 A resolution
Summary for 5I9S
| Entry DOI | 10.2210/pdb5i9s/pdb |
| EMDB information | 8077 |
| Descriptor | Proteinase K, SULFATE ION (3 entities in total) |
| Functional Keywords | hydrolase |
| Biological source | Engyodontium album |
| Total number of polymer chains | 1 |
| Total formula weight | 29150.92 |
| Authors | Hattne, J.,Shi, D.,de la Cruz, M.J.,Reyes, F.E.,Gonen, T. (deposition date: 2016-02-20, release date: 2016-06-08, Last modification date: 2024-11-13) |
| Primary citation | Hattne, J.,Shi, D.,de la Cruz, M.J.,Reyes, F.E.,Gonen, T. Modeling truncated pixel values of faint reflections in MicroED images. J.Appl.Crystallogr., 49:1029-1034, 2016 Cited by PubMed Abstract: The weak pixel counts surrounding the Bragg spots in a diffraction image are important for establishing a model of the background underneath the peak and estimating the reliability of the integrated intensities. Under certain circumstances, particularly with equipment not optimized for low-intensity measurements, these pixel values may be corrupted by corrections applied to the raw image. This can lead to truncation of low pixel counts, resulting in anomalies in the integrated Bragg intensities, such as systematically higher signal-to-noise ratios. A correction for this effect can be approximated by a three-parameter lognormal distribution fitted to the weakly positive-valued pixels at similar scattering angles. The procedure is validated by the improved refinement of an atomic model against structure factor amplitudes derived from corrected micro-electron diffraction (MicroED) images. PubMed: 27275145DOI: 10.1107/S1600576716007196 PDB entries with the same primary citation |
| Experimental method | ELECTRON CRYSTALLOGRAPHY (1.75 Å) |
Structure validation
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