5HYN

Structure of Human Polycomb Repressive Complex 2 (PRC2) with oncogenic histone H3K27M peptide

5HYN の概要

• エントリーDOI: 10.2210/pdb5hyn/pdb
• 分子名称: Histone-lysine N-methyltransferase EZH2, Polycomb protein EED, Polycomb protein SUZ12, ... (7 entities in total)
• 機能のキーワード: chromatin modification complex, transferase
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus : Q15910 O75530 Q15022
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 586815.22

構造登録者

Zhang, Y.,Justin, N.,Wilson, J.R.,Gamblin, S.J. (登録日: 2016-02-01, 公開日: 2016-05-11, 最終更新日: 2025-04-09)

主引用文献

Justin, N.,Zhang, Y.,Tarricone, C.,Martin, S.R.,Chen, S.,Underwood, E.,De Marco, V.,Haire, L.F.,Walker, P.A.,Reinberg, D.,Wilson, J.R.,Gamblin, S.J.
Structural basis of oncogenic histone H3K27M inhibition of human polycomb repressive complex 2.
Nat Commun, 7:11316-11316, 2016

PubMed Abstract: Polycomb repressive complex 2 (PRC2) silences gene expression through trimethylation of K27 of histone H3 (H3K27me3) via its catalytic SET domain. A missense mutation in the substrate of PRC2, histone H3K27M, is associated with certain pediatric brain cancers and is linked to a global decrease of H3K27me3 in the affected cells thought to be mediated by inhibition of PRC2 activity. We present here the crystal structure of human PRC2 in complex with the inhibitory H3K27M peptide bound to the active site of the SET domain, with the methionine residue located in the pocket that normally accommodates the target lysine residue. The structure and binding studies suggest a mechanism for the oncogenic inhibition of H3K27M. The structure also reveals how binding of repressive marks, like H3K27me3, to the EED subunit of the complex leads to enhancement of the catalytic efficiency of the SET domain and thus the propagation of this repressive histone modification.

PubMed: 27121947
DOI: 10.1038/ncomms11316

実験手法

X-RAY DIFFRACTION (2.95 Å)