5HWZ
Crystal structure of nitrophorin 4 D30N mutant with nitrite
Summary for 5HWZ
| Entry DOI | 10.2210/pdb5hwz/pdb |
| Descriptor | Nitrophorin-4, PROTOPORPHYRIN IX CONTAINING FE, NITRITE ION, ... (4 entities in total) |
| Functional Keywords | nitrophorin, nitrite, heme, transport protein |
| Biological source | Rhodnius prolixus (Triatomid bug) |
| Total number of polymer chains | 1 |
| Total formula weight | 20954.17 |
| Authors | Ogata, H.,He, C.,Lubitz, W. (deposition date: 2016-01-29, release date: 2016-05-04, Last modification date: 2024-11-06) |
| Primary citation | He, C.,Ogata, H.,Lubitz, W. Elucidation of the heme active site electronic structure affecting the unprecedented nitrite dismutase activity of the ferrihemebproteins, the nitrophorins. Chem Sci, 7:5332-5340, 2016 Cited by PubMed Abstract: Nitrophorins (NPs) catalyze the nitrite dismutation reaction that is unprecedented in ferriheme proteins. Despite progress in studying the reaction mechanism, fundamental issues regarding the correlation of the structural features with the nitrite dismutase activity of NPs remain elusive. On the other hand, it has been shown that the nitrite complexes of NPs are unique among those of the ferriheme proteins since some of their electron paramagnetic resonance (EPR) spectra show significant highly anisotropic low spin (HALS) signals with large values over 3.2. The origin of HALS signals in ferriheme proteins or models is not well understood, especially in cases where axial ligands other than histidine are present. In this study several mutations were introduced in NP4. The related nitrite coordination and dismutation reaction were investigated. As a result, the EPR spectra of the NP-nitrite complexes were found to be tightly correlated with the extent of heme ruffling and protonation state of the proximal His ligand-dictated by an extended H-bonding network at the heme active site. Furthermore, it is established that the two factors are essential in determining the nitrite dismutase activity of NPs. These results may provide a valuable guide for identifying or designing novel heme proteins with similar activity. PubMed: 30155185DOI: 10.1039/c6sc01019a PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.45 Å) |
Structure validation
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