5HPY

Crystal Structure of RhoA.GDP.MgF3-in complex with human Myosin 9b RhoGAP domain

5HPY の概要

• エントリーDOI: 10.2210/pdb5hpy/pdb
• 分子名称: Unconventional myosin-IXb, Transforming protein RhoA, TRIFLUOROMAGNESATE, ... (6 entities in total)
• 機能のキーワード: complex, rho gtpases, rhogap, gene regulation-signaling protein complex, gene regulation/signaling protein
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Cytoplasm, cell cortex : Q13459; Cell membrane; Lipid-anchor; Cytoplasmic side: P61586
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 94855.84

構造登録者

Yi, F.S.,Ren, J.Q.,Feng, W. (登録日: 2016-01-21, 公開日: 2016-07-13, 最終更新日: 2023-11-08)

主引用文献

Yi, F.,Kong, R.,Ren, J.,Zhu, L.,Lou, J.,Wu, J.Y.,Feng, W.
Noncanonical Myo9b-RhoGAP Accelerates RhoA GTP Hydrolysis by a Dual-Arginine-Finger Mechanism
J.Mol.Biol., 428:3043-3057, 2016

PubMed Abstract: The GTP hydrolysis activities of Rho GTPases are stimulated by GTPase-activating proteins (GAPs), which contain a RhoGAP domain equipped with a characteristic arginine finger and an auxiliary asparagine for catalysis. However, the auxiliary asparagine is missing in the RhoGAP domain of Myo9b (Myo9b-RhoGAP), a unique motorized RhoGAP that specifically targets RhoA for controlling cell motility. Here, we determined the structure of Myo9b-RhoGAP in complex with GDP-bound RhoA and magnesium fluoride. Unexpectedly, Myo9b-RhoGAP contains two arginine fingers at its catalytic site. The first arginine finger resembles the one within the canonical RhoGAP domains and inserts into the nucleotide-binding pocket of RhoA, whereas the second arginine finger anchors the Switch I loop of RhoA and interacts with the nucleotide, stabilizing the transition state of GTP hydrolysis and compensating for the lack of the asparagine. Mutating either of the two arginine fingers impaired the catalytic activity of Myo9b-RhoGAP and affected the Myo9b-mediated cell migration. Our data indicate that Myo9b-RhoGAP accelerates RhoA GTP hydrolysis by a previously unknown dual-arginine-finger mechanism, which may be shared by other noncanonical RhoGAP domains lacking the auxiliary asparagine.

PubMed: 27363609
DOI: 10.1016/j.jmb.2016.06.014

実験手法

X-RAY DIFFRACTION (2.4 Å)