5HMB

Crystal structure of S. sahachiroi AziG

5HMB の概要

• エントリーDOI: 10.2210/pdb5hmb/pdb
• 関連するPDBエントリー: 5HMC
• 分子名称: Azi13, SULFATE ION (3 entities in total)
• 機能のキーワード: azinomycin biosynthesis, polyketide synthase, thioesterase, naphthoate, hydrolase
• 由来する生物種: Streptomyces sahachiroi
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15608.34

構造登録者

Erb, M.S.,Zhang, Y.,Ealick, S.E. (登録日: 2016-01-15, 公開日: 2016-02-03, 最終更新日: 2023-09-27)

主引用文献

Mori, S.,Simkhada, D.,Zhang, H.,Erb, M.S.,Zhang, Y.,Williams, H.,Fedoseyenko, D.,Russell, W.K.,Kim, D.,Fleer, N.,Ealick, S.E.,Watanabe, C.M.
Polyketide Ring Expansion Mediated by a Thioesterase, Chain Elongation and Cyclization Domain, in Azinomycin Biosynthesis: Characterization of AziB and AziG.
Biochemistry, 55:704-714, 2016

PubMed Abstract: The azinomycins are a family of potent antitumor agents with the ability to form interstrand cross-links with DNA. This study reports on the unusual biosynthetic formation of the 5-methyl naphthoate moiety, which is essential for effective DNA association. While sequence analysis predicts that the polyketide synthase (AziB) catalyzes the formation of this naphthoate, 2-methylbenzoic acid, a truncated single-ring product, is formed instead. We demonstrate that the thioesterase (AziG) acts as a chain elongation and cyclization (CEC) domain and is required for the additional two rounds of chain extension to form the expected product.

PubMed: 26731610
DOI: 10.1021/acs.biochem.5b01050

実験手法

X-RAY DIFFRACTION (2.151 Å)