5HMB
Crystal structure of S. sahachiroi AziG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-25 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9183 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 68.466, 68.466, 139.616 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.908 - 2.151 |
| R-factor | 0.2084 |
| Rwork | 0.207 |
| R-free | 0.22890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s4k |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.766 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 5.830 | 2.150 |
| Rmerge | 0.091 | 0.080 | 0.386 |
| Total number of observations | 62888 | ||
| Number of reflections | 9373 | ||
| <I/σ(I)> | 13.9 | ||
| Completeness [%] | 99.5 | 96 | 100 |
| Redundancy | 6.7 | 6.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 1.25 M ammonium sulfate and 0.1 M cacodylate pH 6.0 |
