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5HL4

Acoustic injectors for drop-on-demand serial femtosecond crystallography

Summary for 5HL4
Entry DOI10.2210/pdb5hl4/pdb
Related5F81 5f80
DescriptorRing-hydroxylating dioxygenase, FE2/S2 (INORGANIC) CLUSTER, FE (III) ION, ... (5 entities in total)
Functional Keywordsring-hydroxylating dioxygenase, oxidoreductase
Biological sourceSinorhizobium meliloti 1021 (Ensifer meliloti)
Total number of polymer chains1
Total formula weight46818.24
Authors
Primary citationRoessler, C.G.,Agarwal, R.,Allaire, M.,Alonso-Mori, R.,Andi, B.,Bachega, J.F.,Bommer, M.,Brewster, A.S.,Browne, M.C.,Chatterjee, R.,Cho, E.,Cohen, A.E.,Cowan, M.,Datwani, S.,Davidson, V.L.,Defever, J.,Eaton, B.,Ellson, R.,Feng, Y.,Ghislain, L.P.,Glownia, J.M.,Han, G.,Hattne, J.,Hellmich, J.,Heroux, A.,Ibrahim, M.,Kern, J.,Kuczewski, A.,Lemke, H.T.,Liu, P.,Majlof, L.,McClintock, W.M.,Myers, S.,Nelsen, S.,Olechno, J.,Orville, A.M.,Sauter, N.K.,Soares, A.S.,Soltis, S.M.,Song, H.,Stearns, R.G.,Tran, R.,Tsai, Y.,Uervirojnangkoorn, M.,Wilmot, C.M.,Yachandra, V.,Yano, J.,Yukl, E.T.,Zhu, D.,Zouni, A.
Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography.
Structure, 24:631-640, 2016
Cited by
PubMed Abstract: X-ray free-electron lasers (XFELs) provide very intense X-ray pulses suitable for macromolecular crystallography. Each X-ray pulse typically lasts for tens of femtoseconds and the interval between pulses is many orders of magnitude longer. Here we describe two novel acoustic injection systems that use focused sound waves to eject picoliter to nanoliter crystal-containing droplets out of microplates and into the X-ray pulse from which diffraction data are collected. The on-demand droplet delivery is synchronized to the XFEL pulse scheme, resulting in X-ray pulses intersecting up to 88% of the droplets. We tested several types of samples in a range of crystallization conditions, wherein the overall crystal hit ratio (e.g., fraction of images with observable diffraction patterns) is a function of the microcrystal slurry concentration. We report crystal structures from lysozyme, thermolysin, and stachydrine demethylase (Stc2). Additional samples were screened to demonstrate that these methods can be applied to rare samples.
PubMed: 26996959
DOI: 10.1016/j.str.2016.02.007
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.2 Å)
Structure validation

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