5HHK

Reverse transcriptase domain of group II intron maturase from Roseburia intestinalis in P21 space group (Se-MET)

5HHK の概要

• エントリーDOI: 10.2210/pdb5hhk/pdb
• 関連するPDBエントリー: 5HHJ 5HHL
• 分子名称: Retron-type reverse transcriptase, PHOSPHATE ION, POTASSIUM ION, ... (6 entities in total)
• 機能のキーワード: group ii intron, rna binding protein
• 由来する生物種: Roseburia intestinalis XB6B4
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 72025.80

構造登録者

Zhao, C.,Pyle, A.M. (登録日: 2016-01-11, 公開日: 2016-05-04, 最終更新日: 2019-12-25)

主引用文献

Zhao, C.,Pyle, A.M.
Crystal structures of a group II intron maturase reveal a missing link in spliceosome evolution.
Nat.Struct.Mol.Biol., 23:558-565, 2016

PubMed Abstract: Group II introns are self-splicing ribozymes that are essential in many organisms, and they have been hypothesized to share a common evolutionary ancestor with the spliceosome. Although structural similarity of RNA components supports this connection, it is of interest to determine whether associated protein factors also share an evolutionary heritage. Here we present the crystal structures of reverse transcriptase (RT) domains from two group II intron-encoded proteins (maturases) from Roseburia intestinalis and Eubacterium rectale, obtained at 1.2-Å and 2.1-Å resolution, respectively. These domains are more similar in architecture to the spliceosomal Prp8 RT-like domain than to any other RTs, and they share substantial similarity with flaviviral RNA polymerases. The RT domain itself is sufficient for binding intron RNA with high affinity and specificity, and it is contained within an active RT enzyme. These studies provide a foundation for understanding structure-function relationships within group II intron-maturase complexes.

PubMed: 27136328
DOI: 10.1038/nsmb.3224

実験手法

X-RAY DIFFRACTION (1.4 Å)