5HHF

Crystal structure of Aspergillus fumigatus UDP-Galactopyranose mutase mutant H63A with covalent FAD-Galactopyranose and bound UDP

Summary for 5HHF

• Entry DOI: 10.2210/pdb5hhf/pdb
• Descriptor: UDP-galactopyranose mutase, SULFATE ION, MESO-ERYTHRITOL, ... (7 entities in total)
• Functional Keywords: nucleotide binding, mutase, flavin adenine dinucleotide binding, isomerase, covalent reaction intermediate
• Biological source: Neosartorya fumigata
• Total number of polymer chains: 4
• Total formula weight: 233977.66

Authors

Tanner, J.J. (deposition date: 2016-01-10, release date: 2016-02-17, Last modification date: 2023-09-27)

Primary citation

Mehra-Chaudhary, R.,Dai, Y.,Sobrado, P.,Tanner, J.J.
In Crystallo Capture of a Covalent Intermediate in the UDP-Galactopyranose Mutase Reaction.
Biochemistry, 55:833-836, 2016

PubMed Abstract: UDP-galactopyranose mutase (UGM) plays an essential role in galactofuranose biosynthesis in pathogens by catalyzing the conversion of UDP-galactopyranose to UDP-galactofuranose. Here we report the first crystal structure of a covalent intermediate in the UGM reaction. The 2.3 Å resolution structure reveals UDP bound in the active site and galactopyranose linked to the FAD through a covalent bond between the anomeric C of galactopyranose and N5 of the FAD. The structure confirms the role of the flavin as nucleophile and supports the hypothesis that the proton destined for O5 of galactofuranose is shuttled from N5 of the FAD via O4 of the FAD.

PubMed: 26836146
DOI: 10.1021/acs.biochem.6b00035

Experimental method

X-RAY DIFFRACTION (2.3 Å)