5HGU

Crystal structure of human transcription factor TEAD2 in complex with palmitate

Summary for 5HGU

• Entry DOI: 10.2210/pdb5hgu/pdb
• Related: 3L15
• Descriptor: Transcriptional enhancer factor TEF-4, PALMITIC ACID (3 entities in total)
• Functional Keywords: transcription factor, palmitoylation, hippo pathway, transcription
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 2
• Total formula weight: 54772.16

Authors

Luo, X.,Xiao, H. (deposition date: 2016-01-08, release date: 2016-02-17, Last modification date: 2023-09-27)

Primary citation

Chan, P.,Han, X.,Zheng, B.,DeRan, M.,Yu, J.,Jarugumilli, G.K.,Deng, H.,Pan, D.,Luo, X.,Wu, X.
Autopalmitoylation of TEAD proteins regulates transcriptional output of the Hippo pathway.
Nat.Chem.Biol., 12:282-289, 2016

PubMed Abstract: TEA domain (TEAD) transcription factors bind to the coactivators YAP and TAZ and regulate the transcriptional output of the Hippo pathway, playing critical roles in organ size control and tumorigenesis. Protein S-palmitoylation attaches a fatty acid, palmitate, to cysteine residues and regulates protein trafficking, membrane localization and signaling activities. Using activity-based chemical probes, we discovered that human TEADs possess intrinsic palmitoylating enzyme-like activities and undergo autopalmitoylation at evolutionarily conserved cysteine residues under physiological conditions. We determined the crystal structures of lipid-bound TEADs and found that the lipid chain of palmitate inserts into a conserved deep hydrophobic pocket. Strikingly, palmitoylation did not alter TEAD's localization, but it was required for TEAD's binding to YAP and TAZ and was dispensable for its binding to the Vgll4 tumor suppressor. Moreover, palmitoylation-deficient TEAD mutants impaired TAZ-mediated muscle differentiation in vitro and tissue overgrowth mediated by the Drosophila YAP homolog Yorkie in vivo. Our study directly links autopalmitoylation to the transcriptional regulation of the Hippo pathway.

PubMed: 26900866
DOI: 10.1038/nchembio.2036

Experimental method

X-RAY DIFFRACTION (2.046 Å)