5H98

Crystal structure of Geobacter metallireducens SMUG1

Summary for 5H98

• Entry DOI: 10.2210/pdb5h98/pdb
• Related: 5H93 5H99 5H9I
• Descriptor: Geobacter metallireducens SMUG1 (2 entities in total)
• Functional Keywords: smug1, dna damage, base excision repair, substrate specificity, hydrolase
• Biological source: Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210)
• Total number of polymer chains: 2
• Total formula weight: 52334.03

Authors

Xie, W.,Cao, W.,Zhang, Z.,Shen, J. (deposition date: 2015-12-26, release date: 2016-04-27, Last modification date: 2023-11-08)

Primary citation

Zhang, Z.,Shen, J.,Yang, Y.,Li, J.,Cao, W.,Xie, W.
Structural Basis of Substrate Specificity in Geobacter metallireducens SMUG1
Acs Chem.Biol., 11:1729-1736, 2016

PubMed Abstract: Base deamination is a common type of DNA damage that occurs in all organisms. DNA repair mechanisms are critical to maintain genome integrity, in which the base excision repair pathway plays an essential role. In the BER pathway, the uracil DNA glycosylase superfamily is responsible for removing the deaminated bases from DNA and generates apurinic/apyrimidinic (AP) sites. Geobacter metallireducens SMUG1 (GmeSMUG1) is an interesting family 3 enzyme in the UDG superfamily, with dual substrate specificities for DNA with uracil or xanthine. In contrast, the mutant G63P of GmeSMUG1 has exclusive activity for uracil, while N58D is inactive for both substrates, as we have reported previously. However, the structural bases for these substrate specificities are not well understood. In this study, we solved a series of crystal structures of WT and mutants of GmeSMUG1 at relatively high resolutions. These structures provide insight on the molecular mechanism of xanthine recognition for GmeSMUG1 and indicate that H210 plays a key role in xanthine recognition, which is in good agreement with the results of our EMSA and activity assays. More importantly, our mutant structures allow us to build models to rationalize our previous experimental observations of altered substrate activities of these mutants.

PubMed: 27071000
DOI: 10.1021/acschembio.6b00164

Experimental method

X-RAY DIFFRACTION (2.04 Å)