Cryo-EM structure of the Cdt1-MCM2-7 complex in AMPPNP state

Summary for 5H7I

EMDB information6671 6674 6673 6672
DescriptorDNA replication licensing factor MCM2, DNA replication licensing factor MCM3, DNA replication licensing factor MCM4, ... (7 entities in total)
Functional Keywordshelicase, dna replication, hydrolase
Biological sourceSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Cellular locationNucleus P29469 P24279 P30665 P29496 P53091
Cytoplasm  P38132 P47112
Total number of polymer chains7
Total molecular weight674855.3
Zhai, Y.,Cheng, E.,Wu, H.,Li, N.,Yung, P.Y.,Gao, N.,Tye, B.K. (deposition date: 2016-11-18, release date: 2017-02-08, Last modification date: 2017-03-15)
Primary citation
Zhai, Y.,Cheng, E.,Wu, H.,Li, N.,Yung, P.Y.,Gao, N.,Tye, B.K.
Open-ringed structure of the Cdt1-Mcm2-7 complex as a precursor of the MCM double hexamer
Nat. Struct. Mol. Biol., 24:300-308, 2017
PubMed: 28191894 (PDB entries with the same primary citation)
DOI: 10.1038/nsmb.3374
MImport into Mendeley
Experimental method

Structure validation

ClashscoreRamachandran outliers311.3%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures