5GWM

Solution structure of heterodimeric coiled-coil domain of Drosophila GABAB receptor 1 and 3

> Summary

Summary for 5GWM

DescriptorMetabotropic GABA-B receptor subtype 1, Metabotropic GABA-B receptor subtype 3, isoform A (2 entities in total)
Functional Keywordsgabab receptor, drosophila, coiled-coil, signaling protein
Biological sourceDrosophila melanogaster (Fruit fly)
Total number of polymer chains2
Total molecular weight12281.94
Authors
Liu, X.,Zhang, S.,Zhang, C.X.,Liu, J. (deposition date: 2016-09-12, release date: 2017-09-13)
Primary citation
Liu, X.,Zhang, S.,Zhang, C.X.,Liu, J.
Solution structure of heterodimeric coiled-coil domain of Drosophila GABAB receptor 1 and 3
To Be Published,
Experimental method
SOLUTION NMR
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Structure validation

ClashscoreRamachandran outliersSidechain outliers16014.0%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all NMR structures

More Asymmetric unit images

Molmil generated image of 5gwm
no rotation
Molmil generated image of 5gwm
rotated about x axis by 90°
Molmil generated image of 5gwm
rotated about y axis by 90°

> Structural details

Entity

Chain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
AMetabotropic GABA-B receptor subtype 1polymer536327.11
UniProt (Q9BML7)
Drosophila melanogaster (Fruit fly)
BMetabotropic GABA-B receptor subtype 3, isoform Apolymer505954.81
UniProt (Q9VPS7)
Drosophila melanogaster (Fruit fly)GABAB receptor 3,Metabotropic GABA-B receptor subtype 3,isoform D,isoform E,isoform G

Sequence viewer

Contents of the asymmetric unit

PolymersNumber of chains2
Total molecular weight12281.9
Non-Polymers*Number of molecules0
Total molecular weight0.0
All*Total molecular weight12281.9
*Water molecules are not included.

> Experimental details

Spectrometer

Experimental method:SOLUTION NMR

Spectrometer IDSpectrometer makerSpectrometer modelSpectrometer typeSpectrometer field strength
1AgilentDD2600

Experiment

experiment idconditions idsolution idExperiment type
1112D 1H-15N HSQC
2113D 1H-15N NOESY
3122D 1H-15N HSQC
4122D 1H-13C HSQC
5123D HNCO
13123D HNCACB
14123D CBCA(CO)NH
12123D HBHA(CO)NH
11123D HCCH-TOCSY
10123D 1H-13C NOESY
7123D C(CO)NH

NMR Sample

conditions idNMR sample pHNMR sample pressureNMR sample temperature
171293

Conformers

Conformers Calculated Total Number200
Conformers Submitted Total Number20

> Functional details

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Functional Information from GO Data

ChainGOidnamespacecontents
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Functional Information from PDB Data

site_idNumber of ResiduesDetails
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Functional Information from PDB atom coordinates for the "HETATM" binding sites

site_idNumber of ResiduesDetails
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Functional Information from PROSITE/UniProt

site_idNumber of ResiduesDetails
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Functional Information from SwissProt/UniProt

site_idNumber of ResiduesDetails
SWS_FT_FI1154Helical. {ECO:0000256|SAM:Phobius}.
ChainResidueDetails
ANA*
ANA*
ANA*
ANA*
ANA*
ANA*
ANA*

SWS_FT_FI2131Helical. {ECO:0000256|SAM:Phobius}.
ChainResidueDetails
BNA*
BNA*
BNA*
BNA*
BNA*
BNA*

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Catalytic Information from CSA

site_idNumber of ResiduesDetails

> Sequence Neighbor

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