5GW5

Structure of TRiC-AMP-PNP

> Summary

Summary for 5GW5

Related5GW4
EMDB informationEMD-9541
DescriptorT-complex protein 1 subunit alpha, T-complex protein 1 subunit beta, T-complex protein 1 subunit delta, T-complex protein 1 subunit epsilon, T-complex protein 1 subunit gamma, T-complex protein 1 subunit eta, T-complex protein 1 subunit theta, T-complex protein 1 subunit zeta
Functional Keywordschaperonin, yeast, chaperone
Biological sourceSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Cellular locationCytoplasm P12612 P39076 P39078 P40413 P39077 P42943 P47079 P39079
Total number of polymer chains16
Total molecular weight955870.85
Authors
Zang, Y.,Jin, M.,Wang, H.,Cong, Y. (deposition date: 2016-09-08, release date: 2016-10-26)
Primary citation
Zang, Y.,Jin, M.,Wang, H.,Cui, Z.,Kong, L.,Liu, C.,Cong, Y.
Staggered ATP-binding mechanism of eukaryotic chaperonin TRiC/CCT revealed by high-resolution cryo-EM.
Nat.Struct.Mol.Biol., 2016
DOI: 10.1038/nsmb.3309
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
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Structure validation

ClashscoreRamachandran outliersSidechain outliers60.1%0.0%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 5gw5
no rotation
Molmil generated image of 5gw5
rotated about x axis by 90°
Molmil generated image of 5gw5
rotated about y axis by 90°

> Structural details

Entity

Chain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
a, AT-complex protein 1 subunit alphapolymer55960557.62
UniProt (P12612)
Pfam (PF00118)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)TCP-1-alpha,CCT-alpha
b, BT-complex protein 1 subunit betapolymer52757276.32
UniProt (P39076)
Pfam (PF00118)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)TCP-1-beta,CCT-beta
d, DT-complex protein 1 subunit deltapolymer52857682.42
UniProt (P39078)
Pfam (PF00118)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)TCP-1-delta,CCT-delta
e, ET-complex protein 1 subunit epsilonpolymer56261995.02
UniProt (P40413)
Pfam (PF00118)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)TCP-1-epsilon,CCT-epsilon
g, GT-complex protein 1 subunit gammapolymer53458889.12
UniProt (P39077)
Pfam (PF00118)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)TCP-1-gamma,CCT-gamma
h, HT-complex protein 1 subunit etapolymer55059802.42
UniProt (P42943)
Pfam (PF00118)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)TCP-1-eta,CCT-eta
q, QT-complex protein 1 subunit thetapolymer56861735.12
UniProt (P47079)
Pfam (PF00118)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)TCP-1-theta,CCT-theta
z, ZT-complex protein 1 subunit zetapolymer54659997.62
UniProt (P39079)
Pfam (PF00118)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)TCP-1-zeta,CCT-zeta

Sequence viewer

Contents of the asymmetric unit

PolymersNumber of chains16
Total molecular weight955870.9
Non-Polymers*Number of molecules0
Total molecular weight0.0
All*Total molecular weight955870.9
*Water molecules are not included.

> Experimental details

Refinement Statistics

Experimental method:ELECTRON MICROSCOPY

Resolution limits -
the highest resolution shell value -
RMSD bond length0.002
RMSD bond angle0.416

Data Collection Statistics

Resolution limits -
the highest resolution shell value -

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
Annotated Information is extracted from Literature Info*

> Functional details

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Functional Information from GO Data

ChainGOidnamespacecontents
A0005832cellular_componentchaperonin-containing T-complex
A0005524molecular_functionATP binding
A0044183molecular_functionprotein binding involved in protein folding
A0051082molecular_functionunfolded protein binding
A0006458biological_process'de novo' protein folding
A0061077biological_processchaperone-mediated protein folding
A0006457biological_processprotein folding
a0005832cellular_componentchaperonin-containing T-complex
a0005524molecular_functionATP binding
a0044183molecular_functionprotein binding involved in protein folding
a0051082molecular_functionunfolded protein binding
a0006458biological_process'de novo' protein folding
a0061077biological_processchaperone-mediated protein folding
a0006457biological_processprotein folding
B0046658cellular_componentanchored component of plasma membrane
B0005618cellular_componentcell wall
B0005832cellular_componentchaperonin-containing T-complex
B0005524molecular_functionATP binding
B0044183molecular_functionprotein binding involved in protein folding
B0051082molecular_functionunfolded protein binding
B0006458biological_process'de novo' protein folding
B0061077biological_processchaperone-mediated protein folding
B0006457biological_processprotein folding
b0046658cellular_componentanchored component of plasma membrane
b0005618cellular_componentcell wall
b0005832cellular_componentchaperonin-containing T-complex
b0005524molecular_functionATP binding
b0044183molecular_functionprotein binding involved in protein folding
b0051082molecular_functionunfolded protein binding
b0006458biological_process'de novo' protein folding
b0061077biological_processchaperone-mediated protein folding
b0006457biological_processprotein folding
D0005832cellular_componentchaperonin-containing T-complex
D0005524molecular_functionATP binding
D0044183molecular_functionprotein binding involved in protein folding
D0051082molecular_functionunfolded protein binding
D0006458biological_process'de novo' protein folding
D0061077biological_processchaperone-mediated protein folding
D0006457biological_processprotein folding
D0046686biological_processresponse to cadmium ion
d0005832cellular_componentchaperonin-containing T-complex
d0005524molecular_functionATP binding
d0044183molecular_functionprotein binding involved in protein folding
d0051082molecular_functionunfolded protein binding
d0006458biological_process'de novo' protein folding
d0061077biological_processchaperone-mediated protein folding
d0006457biological_processprotein folding
d0046686biological_processresponse to cadmium ion
E0005832cellular_componentchaperonin-containing T-complex
E0005886cellular_componentplasma membrane
E0005524molecular_functionATP binding
E0044183molecular_functionprotein binding involved in protein folding
E0051082molecular_functionunfolded protein binding
E0006458biological_process'de novo' protein folding
E0061077biological_processchaperone-mediated protein folding
E0006457biological_processprotein folding
e0005832cellular_componentchaperonin-containing T-complex
e0005886cellular_componentplasma membrane
e0005524molecular_functionATP binding
e0044183molecular_functionprotein binding involved in protein folding
e0051082molecular_functionunfolded protein binding
e0006458biological_process'de novo' protein folding
e0061077biological_processchaperone-mediated protein folding
e0006457biological_processprotein folding
G0005832cellular_componentchaperonin-containing T-complex
G0005524molecular_functionATP binding
G0044183molecular_functionprotein binding involved in protein folding
G0051082molecular_functionunfolded protein binding
G0006458biological_process'de novo' protein folding
G0061077biological_processchaperone-mediated protein folding
G0006457biological_processprotein folding
g0005832cellular_componentchaperonin-containing T-complex
g0005524molecular_functionATP binding
g0044183molecular_functionprotein binding involved in protein folding
g0051082molecular_functionunfolded protein binding
g0006458biological_process'de novo' protein folding
g0061077biological_processchaperone-mediated protein folding
g0006457biological_processprotein folding
H0005832cellular_componentchaperonin-containing T-complex
H0005524molecular_functionATP binding
H0044183molecular_functionprotein binding involved in protein folding
H0051082molecular_functionunfolded protein binding
H0006458biological_process'de novo' protein folding
H0061077biological_processchaperone-mediated protein folding
H0006457biological_processprotein folding
H0046686biological_processresponse to cadmium ion
h0005832cellular_componentchaperonin-containing T-complex
h0005524molecular_functionATP binding
h0044183molecular_functionprotein binding involved in protein folding
h0051082molecular_functionunfolded protein binding
h0006458biological_process'de novo' protein folding
h0061077biological_processchaperone-mediated protein folding
h0006457biological_processprotein folding
h0046686biological_processresponse to cadmium ion
Q0005832cellular_componentchaperonin-containing T-complex
Q0016020cellular_componentmembrane
Q0005524molecular_functionATP binding
Q0044183molecular_functionprotein binding involved in protein folding
Q0051082molecular_functionunfolded protein binding
Q0006458biological_process'de novo' protein folding
Q0061077biological_processchaperone-mediated protein folding
Q0051050biological_processpositive regulation of transport
Q0006457biological_processprotein folding
q0005832cellular_componentchaperonin-containing T-complex
q0016020cellular_componentmembrane
q0005524molecular_functionATP binding
q0044183molecular_functionprotein binding involved in protein folding
q0051082molecular_functionunfolded protein binding
q0006458biological_process'de novo' protein folding
q0061077biological_processchaperone-mediated protein folding
q0051050biological_processpositive regulation of transport
q0006457biological_processprotein folding
Z0005832cellular_componentchaperonin-containing T-complex
Z0005524molecular_functionATP binding
Z0044183molecular_functionprotein binding involved in protein folding
Z0051082molecular_functionunfolded protein binding
Z0006458biological_process'de novo' protein folding
Z0061077biological_processchaperone-mediated protein folding
Z0006457biological_processprotein folding
z0005832cellular_componentchaperonin-containing T-complex
z0005524molecular_functionATP binding
z0044183molecular_functionprotein binding involved in protein folding
z0051082molecular_functionunfolded protein binding
z0006458biological_process'de novo' protein folding
z0061077biological_processchaperone-mediated protein folding
z0006457biological_processprotein folding
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Functional Information from PDB Data

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Functional Information from PDB atom coordinates for the "HETATM" binding sites

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Functional Information from PROSITE/UniProt

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Functional Information from SwissProt/UniProt

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Catalytic Information from CSA

site_idNumber of ResiduesDetails

> Sequence Neighbor

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