5GU9

Structure of biotin carboxyl carrier protein from pyrococcus horikoshi OT3 (delta N79) A138I mutant

Summary for 5GU9

• Entry DOI: 10.2210/pdb5gu9/pdb
• Related: 5GU8 5GUA
• Descriptor: 149aa long hypothetical methylmalonyl-CoA decarboxylase gamma chain (2 entities in total)
• Functional Keywords: surface engineering, crystal packing, crystal contact engineering, transferase
• Biological source: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
• Total number of polymer chains: 1
• Total formula weight: 7742.20

Authors

Yamada, K.,Kunishima, N.,Matsuura, Y.,Nakai, K.,Naitow, H.,Fukasawa, Y.,Tomii, K. (deposition date: 2016-08-26, release date: 2017-08-30, Last modification date: 2023-11-08)

Primary citation

Yamada, K.D.,Kunishima, N.,Matsuura, Y.,Nakai, K.,Naitow, H.,Fukasawa, Y.,Tomii, K.
Designing better diffracting crystals of biotin carboxyl carrier protein from Pyrococcus horikoshii by a mutation based on the crystal-packing propensity of amino acids.
Acta Crystallogr D Struct Biol, 73:757-766, 2017

PubMed Abstract: An alternative rational approach to improve protein crystals by using single-site mutation of surface residues is proposed based on the results of a statistical analysis using a compiled data set of 918 independent crystal structures, thereby reflecting not only the entropic effect but also other effects upon protein crystallization. This analysis reveals a clear difference in the crystal-packing propensity of amino acids depending on the secondary-structural class. To verify this result, a systematic crystallization experiment was performed with the biotin carboxyl carrier protein from Pyrococcus horikoshii OT3 (PhBCCP). Six single-site mutations were examined: Ala138 on the surface of a β-sheet was mutated to Ile, Tyr, Arg, Gln, Val and Lys. In agreement with prediction, it was observed that the two mutants (A138I and A138Y) harbouring the residues with the highest crystal-packing propensities for β-sheet at position 138 provided better crystallization scores relative to those of other constructs, including the wild type, and that the crystal-packing propensity for β-sheet provided the best correlation with the ratio of obtaining crystals. Two new crystal forms of these mutants were obtained that diffracted to high resolution, generating novel packing interfaces with the mutated residues (Ile/Tyr). The mutations introduced did not affect the overall structures, indicating that a β-sheet can accommodate a successful mutation if it is carefully selected so as to avoid intramolecular steric hindrance. A significant negative correlation between the ratio of obtaining amorphous precipitate and the crystal-packing propensity was also found.

PubMed: 28876239
DOI: 10.1107/S2059798317010932

Experimental method

X-RAY DIFFRACTION (1.9 Å)