5GU0
Crystal structure of Au.CL-apo-E45C/R52C-rHLFr
Summary for 5GU0
| Entry DOI | 10.2210/pdb5gu0/pdb |
| Related | 5GU1 5GU2 5GU3 |
| Descriptor | Ferritin light chain, GOLD ION, CADMIUM ION, ... (6 entities in total) |
| Functional Keywords | iron storage, metal binding protein |
| Biological source | Equus caballus (Horse) |
| Total number of polymer chains | 1 |
| Total formula weight | 22021.64 |
| Authors | |
| Primary citation | Maity, B.,Abe, S.,Ueno, T. Observation of gold sub-nanocluster nucleation within a crystalline protein cage Nat Commun, 8:14820-14820, 2017 Cited by PubMed Abstract: Protein scaffolds provide unique metal coordination environments that promote biomineralization processes. It is expected that protein scaffolds can be developed to prepare inorganic nanomaterials with important biomedical and material applications. Despite many promising applications, it remains challenging to elucidate the detailed mechanisms of formation of metal nanoparticles in protein environments. In the present work, we describe a crystalline protein cage constructed by crosslinking treatment of a single crystal of apo-ferritin for structural characterization of the formation of sub-nanocluster with reduction reaction. The crystal structure analysis shows the gradual movement of the Au ions towards the centre of the three-fold symmetric channels of the protein cage to form a sub-nanocluster with accompanying significant conformational changes of the amino-acid residues bound to Au ions during the process. These results contribute to our understanding of metal core formation as well as interactions of the metal core with the protein environment. PubMed: 28300064DOI: 10.1038/ncomms14820 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.95 Å) |
Structure validation
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