5GTB

crystal structure of intermembrane space region of the ARC6-PDV2 complex

Summary for 5GTB

• Entry DOI: 10.2210/pdb5gtb/pdb
• Descriptor: Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 6, chloroplastic, Plastid division protein PDV2 (2 entities in total)
• Functional Keywords: beta barrel, key in the lock, complex, plant protein
• Biological source: Arabidopsis thaliana (Mouse-ear cress); More
• Cellular location: Plastid, chloroplast inner membrane ; Single-pass membrane protein : Q9FIG9; Plastid, chloroplast outer membrane ; Single-pass membrane protein : Q9XII1
• Total number of polymer chains: 2
• Total formula weight: 20065.63

Authors

Feng, Y.,Wang, W. (deposition date: 2016-08-19, release date: 2017-03-01, Last modification date: 2023-11-08)

Primary citation

Wang, W.,Li, J.,Sun, Q.,Yu, X.,Zhang, W.,Jia, N.,An, C.,Li, Y.,Dong, Y.,Han, F.,Chang, N.,Liu, X.,Zhu, Z.,Yu, Y.,Fan, S.,Yang, M.,Luo, S.Z.,Gao, H.,Feng, Y.
Structural insights into the coordination of plastid division by the ARC6-PDV2 complex
Nat Plants, 3:17011-17011, 2017

PubMed Abstract: Chloroplasts divide by binary fission, which is accomplished by the simultaneous constriction of the FtsZ ring on the stromal side of the inner envelope membrane, and the ARC5 ring on the cytosolic side of the outer envelope membrane. The two rings are connected and coordinated mainly by the interaction between the inner envelope membrane protein ARC6 and the outer envelope membrane protein PDV2 in the intermembrane space. The underlying mechanism of this coordination is unclear to date. Here, we solved the crystal structure of the intermembrane space region of the ARC6-PDV2 complex. The results indicated that PDV2 inserts its carboxy terminus into a pocket formed in ARC6, and this interaction further induces the dimerization of the intermembrane space regions of two ARC6 molecules. A pdv2 mutant attenuating PDV2-induced ARC6 dimerization showed abnormal morphology of ARC6 rings and compromised chloroplast division in plant cells. Together, our data reveal that PDV2-induced dimerization of ARC6 plays a critical role in chloroplast division and provide insights into the coordination mechanism of the internal and external plastid division machineries.

PubMed: 28248291
DOI: 10.1038/nplants.2017.11

Experimental method

X-RAY DIFFRACTION (2.871 Å)