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5GSZ

Crystal Structure of the KIF19A Motor Domain Complexed with Mg-ADP

Summary for 5GSZ
Entry DOI10.2210/pdb5gsz/pdb
DescriptorKinesin-like protein KIF19, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
Functional Keywordskinesin, motor domain, mg-adp, motor protein
Biological sourceMus musculus (Mouse)
Total number of polymer chains1
Total formula weight40120.52
Authors
Wang, D.,Nitta, R.,Hirokawa, N. (deposition date: 2016-08-18, release date: 2016-09-28, Last modification date: 2024-10-16)
Primary citationWang, D.,Nitta, R.,Morikawa, M.,Yajima, H.,Inoue, S.,Shigematsu, H.,Kikkawa, M.,Hirokawa, N.
Motility and microtubule depolymerization mechanisms of the Kinesin-8 motor, KIF19A
Elife, 5:-, 2016
Cited by
PubMed Abstract: The kinesin-8 motor, KIF19A, accumulates at cilia tips and controls cilium length. Defective KIF19A leads to hydrocephalus and female infertility because of abnormally elongated cilia. Uniquely among kinesins, KIF19A possesses the dual functions of motility along ciliary microtubules and depolymerization of microtubules. To elucidate the molecular mechanisms of these functions we solved the crystal structure of its motor domain and determined its cryo-electron microscopy structure complexed with a microtubule. The features of KIF19A that enable its dual function are clustered on its microtubule-binding side. Unexpectedly, a destabilized switch II coordinates with a destabilized L8 to enable KIF19A to adjust to both straight and curved microtubule protofilaments. The basic clusters of L2 and L12 tether the microtubule. The long L2 with a characteristic acidic-hydrophobic-basic sequence effectively stabilizes the curved conformation of microtubule ends. Hence, KIF19A utilizes multiple strategies to accomplish the dual functions of motility and microtubule depolymerization by ATP hydrolysis.
PubMed: 27690357
DOI: 10.7554/eLife.18101
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.72 Å)
Structure validation

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