5GPE

Crystal structure of the transcription regulator PbrR691 from Ralstonia metallidurans CH34 in complex with Lead(II)

Summary for 5GPE

• Entry DOI: 10.2210/pdb5gpe/pdb
• Descriptor: Transcriptional regulator, MerR-family, LEAD (II) ION (3 entities in total)
• Functional Keywords: pbrr691, merr family, transcription activator, transcription
• Biological source: Cupriavidus metallidurans CH34
• Total number of polymer chains: 8
• Total formula weight: 121324.66

Authors

Huang, S.Q.,Chen, W.Z.,Wang, D.,Hu, Q.Y.,Liu, X.C.,Gan, J.H.,Chen, H. (deposition date: 2016-08-01, release date: 2016-12-28, Last modification date: 2023-11-08)

Primary citation

Huang, S.Q.,Liu, X.C.,Wang, D.,Chen, W.Z.,Hu, Q.Y.,Wei, T.B.,Zhou, W.Q.,Gan, J.H.,Chen, H.
Structural Basis for the Selective Pb(II) Recognition of Metalloregulatory Protein PbrR691
Inorg Chem, 55:12516-12519, 2016

PubMed Abstract: The transcription regulator PbrR691, one of the MerR family proteins, shows extremely high sensitivity and selectivity toward Pb(II) in Ralstonia metallidurans CH34. Here, we present the crystal structure of PbrR691 in complex with Pb(II) at 2.0 Å resolution. The Pb(II) coordinates with three conserved cysteines and adopts a unique trigonal-pyramidal (hemidirected) geometry. To our knowledge, the PbrR691-Pb(II) structure provides the first three-dimensional visualization of a functional hemidirected lead(II) thiolate coordinate geometry in a protein.

PubMed: 27989185
DOI: 10.1021/acs.inorgchem.6b02397

Experimental method

X-RAY DIFFRACTION (2.01 Å)