5GN8

Structure of a 48-mer protein nanocage fabricated from its 24-mer analogue by subunit interface redesign

Summary for 5GN8

• Entry DOI: 10.2210/pdb5gn8/pdb
• Descriptor: Ferritin heavy chain, CALCIUM ION, ... (4 entities in total)
• Functional Keywords: ferritin, nanocage, subunit interface redesign, oxidoreductase
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 37705.05

Authors

Zhang, S.,Zang, J.,Zhao, G.,Mikami, B. (deposition date: 2016-07-19, release date: 2016-12-14, Last modification date: 2024-11-06)

Primary citation

Zhang, S.,Zang, J.,Zhang, X.,Chen, H.,Mikami, B.,Zhao, G.
"Silent" Amino Acid Residues at Key Subunit Interfaces Regulate the Geometry of Protein Nanocages
ACS Nano, 10:10382-10388, 2016

PubMed Abstract: Rendering the geometry of protein-based assemblies controllable remains challenging. Protein shell-like nanocages represent particularly interesting targets for designed assembly. Here, we introduce an engineering strategy-key subunit interface redesign (KSIR)-that alters a natural subunit-subunit interface by selective deletion of a small number of "silent" amino acid residues (no participation in interfacial interactions) into one that triggers the generation of a non-native protein cage. We have applied KSIR to construct a non-native 48-mer nanocage from its native 24-mer recombinant human H-chain ferritin (rHuHF). This protein is a heteropolymer composed of equal numbers of two different subunits which are derived from one polypeptide. This strategy has allowed the study of conversion between protein nanocages with different geometries by re-engineering key subunit interfaces and the demonstration of the important role of the above-mentioned specific residues in providing geometric specificity for protein assembly.

PubMed: 27934076
DOI: 10.1021/acsnano.6b06235

Experimental method

X-RAY DIFFRACTION (2.805 Å)