5GN8

Structure of a 48-mer protein nanocage fabricated from its 24-mer analogue by subunit interface redesign

5GN8 の概要

• エントリーDOI: 10.2210/pdb5gn8/pdb
• 分子名称: Ferritin heavy chain, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: ferritin, nanocage, subunit interface redesign, oxidoreductase
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37705.05

構造登録者

Zhang, S.,Zang, J.,Zhao, G.,Mikami, B. (登録日: 2016-07-19, 公開日: 2016-12-14, 最終更新日: 2024-11-06)

主引用文献

Zhang, S.,Zang, J.,Zhang, X.,Chen, H.,Mikami, B.,Zhao, G.
"Silent" Amino Acid Residues at Key Subunit Interfaces Regulate the Geometry of Protein Nanocages
ACS Nano, 10:10382-10388, 2016

PubMed Abstract: Rendering the geometry of protein-based assemblies controllable remains challenging. Protein shell-like nanocages represent particularly interesting targets for designed assembly. Here, we introduce an engineering strategy-key subunit interface redesign (KSIR)-that alters a natural subunit-subunit interface by selective deletion of a small number of "silent" amino acid residues (no participation in interfacial interactions) into one that triggers the generation of a non-native protein cage. We have applied KSIR to construct a non-native 48-mer nanocage from its native 24-mer recombinant human H-chain ferritin (rHuHF). This protein is a heteropolymer composed of equal numbers of two different subunits which are derived from one polypeptide. This strategy has allowed the study of conversion between protein nanocages with different geometries by re-engineering key subunit interfaces and the demonstration of the important role of the above-mentioned specific residues in providing geometric specificity for protein assembly.

PubMed: 27934076
DOI: 10.1021/acsnano.6b06235

実験手法

X-RAY DIFFRACTION (2.805 Å)