5GN5

Crystal structure of glycerol kinase from Trypanosoma brucei gambiense complexed with cumarin derivative-17

5GN5 の概要

• エントリーDOI: 10.2210/pdb5gn5/pdb
• 関連するPDBエントリー: 3WXI 5GN6 5GN7 5GN9
• 分子名称: Glycerol kinase, 4-[[4-(4-methoxyphenyl)piperazin-1-yl]methyl]-7,8-bis(oxidanyl)chromen-2-one, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: glycerol kinase, inhibitor, drug, african trypanosomes, transferase
• 由来する生物種: Trypanosoma brucei gambiense
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 228322.52

構造登録者

Balogun, E.O.,Inaoka, D.K.,Shiba, T.,Tsuge, T.,May, B.,Sato, T.,Kido, Y.,Takeshi, N.,Aoki, T.,Honma, T.,Tanaka, A.,Inoue, M.,Matsuoka, S.,Michels, P.A.M.,Watanabe, Y.,Moore, A.L.,Harada, S.,Kita, K. (登録日: 2016-07-19, 公開日: 2017-07-26, 最終更新日: 2023-11-08)

主引用文献

Balogun, E.O.,Inaoka, D.K.,Shiba, T.,Tsuge, C.,May, B.,Sato, T.,Kido, Y.,Nara, T.,Aoki, T.,Honma, T.,Tanaka, A.,Inoue, M.,Matsuoka, S.,Michels, P.A.M.,Watanabe, Y.I.,Moore, A.L.,Harada, S.,Kita, K.
Discovery of trypanocidal coumarins with dual inhibition of both the glycerol kinase and alternative oxidase ofTrypanosoma brucei brucei.
Faseb J., 33:13002-13013, 2019

PubMed Abstract: African trypanosomiasis, sleeping sickness in humans or nagana in animals, is a potentially fatal neglected tropical disease and a threat to 65 million human lives and 100 million small and large livestock animals in sub-Saharan Africa. Available treatments for this devastating disease are few and have limited efficacy, prompting the search for new drug candidates. Simultaneous inhibition of the trypanosomal glycerol kinase (TGK) and trypanosomal alternative oxidase (TAO) is considered a validated strategy toward the development of new drugs. Our goal is to develop a TGK-specific inhibitor for coadministration with ascofuranone (AF), the most potent TAO inhibitor. Here, we report on the identification of novel compounds with inhibitory potency against TGK. Importantly, one of these compounds (compound 17) and its derivatives (17a and 17b) killed trypanosomes even in the absence of AF. Inhibition kinetics revealed that derivative 17b is a mixed-type and competitive inhibitor for TGK and TAO, respectively. Structural data revealed the molecular basis of this dual inhibitory action, which, in our opinion, will aid in the successful development of a promising drug to treat trypanosomiasis. Although the EC of compound 17b against trypanosome cells was 1.77 µM, it had no effect on cultured human cells, even at 50 µM.-Balogun, E. O., Inaoka, D. K., Shiba, T., Tsuge, C., May, B., Sato, T., Kido, Y., Nara, T., Aoki, T., Honma, T., Tanaka, A., Inoue, M., Matsuoka, S., Michels, P. A. M., Watanabe, Y.-I., Moore, A. L., Harada, S., Kita, K. Discovery of trypanocidal coumarins with dual inhibition of both the glycerol kinase and alternative oxidase of

PubMed: 31525300
DOI: 10.1096/fj.201901342R

実験手法

X-RAY DIFFRACTION (2.85 Å)