5GN0

Structure of TAZ-TEAD complex

Summary for 5GN0

• Entry DOI: 10.2210/pdb5gn0/pdb
• Descriptor: Transcriptional enhancer factor TEF-3, WW domain-containing transcription regulator protein 1, CITRIC ACID, ... (5 entities in total)
• Functional Keywords: transcription
• Biological source: Mus musculus (Mouse); More
• Cellular location: Nucleus: Q62296 Q9EPK5
• Total number of polymer chains: 8
• Total formula weight: 123151.67

Authors

Kaan, H.Y.K.,Song, H. (deposition date: 2016-07-18, release date: 2017-05-31, Last modification date: 2024-11-20)

Primary citation

Kaan, H.Y.K.,Chan, S.W.,Tan, S.K.J.,Guo, F.,Lim, C.J.,Hong, W.,Song, H.
Crystal structure of TAZ-TEAD complex reveals a distinct interaction mode from that of YAP-TEAD complex
Sci Rep, 7:2035-2035, 2017

PubMed Abstract: The Hippo pathway is a tumor suppressor pathway that is implicated in the regulation of organ size. The pathway has three components: the upstream regulatory factors, the kinase core, and the downstream transcriptional machinery, which consists of YAP, TAZ (transcription co-activators) and TEAD (transcription factor). Formation of YAP/TAZ-TEAD complexes leads to the transcription of growth-promoting genes. Herein, we report the crystal structure of TAZ-TEAD4 complex, which reveals two binding modes. The first is similar to the published YAP-TEAD structure. The second is a unique binding mode, whereby two molecules of TAZ bind to and bridge two molecules of TEAD4. We validated the latter using cross-linking and multi-angle light scattering. Using siRNA, we showed that TAZ knockdown leads to a decrease in TEAD4 dimerization. Lastly, results from luciferase assays, using YAP/TAZ transfected or knockdown cells, give support to the non-redundancy of YAP/TAZ co-activators in regulating gene expression in the Hippo pathway.

PubMed: 28515457
DOI: 10.1038/s41598-017-02219-9

Experimental method

X-RAY DIFFRACTION (2.9 Å)