5FW5

Crystal structure of human G3BP1 in complex with Semliki Forest Virus nsP3-25 comprising two FGDF motives

Summary for 5FW5

• Entry DOI: 10.2210/pdb5fw5/pdb
• Descriptor: RAS GTPASE-ACTIVATING PROTEIN-BINDING PROTEIN 1, NON-STRUCTURAL PROTEIN 3, SULFATE ION, ... (7 entities in total)
• Functional Keywords: hydrolase, transferase, nonstructural protein 3 (nsp3), ras-gtpase activating protein sh3 domain binding protein g3bp1, rasputin, stress granule associated
• Biological source: HOMO SAPIENS (HUMAN); More
• Cellular location: Cytoplasm: Q13283; Non-structural polyprotein: Host endosome membrane; Peripheral membrane protein; Cytoplasmic side. P123: Host endosome membrane; Peripheral membrane protein; Cytoplasmic side. mRNA-capping enzyme nsP1: Host endosome membrane; Peripheral membrane protein; Cytoplasmic side. Protease nsP2: Host endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Non-structural protein 3: Host endosome membrane; Peripheral membrane protein; Cytoplasmic side. RNA-directed RNA polymerase nsP4: Host endosome membrane; Peripheral membrane protein; Cytoplasmic side: P08411
• Total number of polymer chains: 3
• Total formula weight: 35656.03

Authors

Schulte, T.,Liu, L.,Panas, M.D.,Thaa, B.,Goette, B.,Achour, A.,McInerney, G.M. (deposition date: 2016-02-12, release date: 2016-07-20, Last modification date: 2024-01-10)

• Primary citation: Schulte, T.,Liu, L.,Panas, M.D.,Thaa, B.,Dickson, N.,Gotte, B.,Achour, A.,McInerney, G.M.; Combined structural, biochemical and cellular evidence demonstrates that both FGDF motifs in alphavirus nsP3 are required for efficient replication.; Open Biol, 6:-, 2016; PubMed: 27383630; DOI: 10.1098/rsob.160078

Experimental method

X-RAY DIFFRACTION (1.92 Å)