5FVA

Toscana Virus Nucleocapsid Protein

5FVA の概要

• エントリーDOI: 10.2210/pdb5fva/pdb
• 分子名称: NUCLEOPROTEIN, SODIUM ION (3 entities in total)
• 機能のキーワード: viral protein, toscana virus, nucleocapside, nucleoprotein, phlebovirus, rna virus, bunyaviridae
• 由来する生物種: TOSCANA VIRUS
• 細胞内の位置: Virion: P21701
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 166110.75

構造登録者

Baklouti, A.,Sevajol, M.,Goulet, A.,Lichiere, J.,Ferron, F.,Canard, B.,Charrel, R.N.,Coutard, B.,Papageorgiou, N. (登録日: 2016-02-03, 公開日: 2016-03-09, 最終更新日: 2024-01-10)

主引用文献

Baklouti, A.,Goulet, A.,Lichiere, J.,Canard, B.,Charrel, R.N.,Ferron, F.,Coutard, B.,Papageorgiou, N.
Toscana virus nucleoprotein oligomer organization observed in solution.
Acta Crystallogr D Struct Biol, 73:650-659, 2017

PubMed Abstract: Toscana virus (TOSV) is an arthropod-borne virus belonging to the Phlebovirus genus within the Bunyaviridae family. As in other bunyaviruses, the genome of TOSV is made up of three RNA segments. They are encapsidated by the nucleoprotein (N), which also plays an essential role in virus replication. To date, crystallographic structures of phlebovirus N have systematically revealed closed-ring organizations which do not fully match the filamentous organization of the ribonucleoprotein (RNP) complex observed by electron microscopy. In order to further bridge the gap between crystallographic data on N and observations of the RNP by electron microscopy, the structural organization of recombinant TOSV N was investigated by an integrative approach combining X-ray diffraction crystallography, transmission electron microscopy, small-angle X-ray scattering, size-exclusion chromatography and multi-angle laser light scattering. It was found that in solution TOSV N forms open oligomers consistent with the encapsidation mechanism of phlebovirus RNA.

PubMed: 28777080
DOI: 10.1107/S2059798317008774

実験手法

X-RAY DIFFRACTION (3.7 Å)