5FNP

High resolution Zn containing Iron sulfur cluster repair protein YtfE

Summary for 5FNP

• Entry DOI: 10.2210/pdb5fnp/pdb
• Related: 5FNN 5FNY
• Descriptor: IRON-SULFUR CLUSTER REPAIR PROTEIN YTFE, ZINC ION, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: oxidoreductase, nitric oxide, iron-sulfur cluster repair, di-fe center.
• Biological source: ESCHERICHIA COLI
• Cellular location: Cytoplasm : P69506
• Total number of polymer chains: 2
• Total formula weight: 50067.57

Authors

Lo, F.-C.,Hsieh, C.-C.,Maestre-Reyna, M.,Chen, C.-Y.,Ko, T.-P.,Horng, Y.-C.,Lai, Y.-C.,Chiang, Y.-W.,Chou, C.-M.,Chiang, C.-H.,Huang, W.-N.,Liaw, W.-F. (deposition date: 2015-11-16, release date: 2016-04-27, Last modification date: 2024-01-10)

Primary citation

Lo, F.-C.,Hsieh, C.-C.,Maestre-Reyna, M.,Chen, C.-Y.,Ko, T.-P.,Horng, Y.-C.,Lai, Y.-C.,Chiang, Y.-W.,Chou, C.-M.,Chiang, C.-H.,Huang, W.-N.,Liaw, W.-F.
Crystal Structure of the Repair of Iron Centers Protein Ytfe and its Interaction with No
Chemistry, 22:9768-, 2016

PubMed Abstract: Molecular mechanisms underlying the repair of nitrosylated [Fe-S] clusters by the microbial protein YtfE remain poorly understood. The X-ray crystal structure of YtfE, in combination with EPR, magnetic circular dichroism (MCD), UV, and (17) O-labeling electron spin echo envelope modulation measurements, show that each iron of the oxo-bridged Fe(II) -Fe(III) diiron core is coordinatively unsaturated with each iron bound to two bridging carboxylates and two terminal histidines in addition to an oxo-bridge. Structural analysis reveals that there are two solvent-accessible tunnels, both of which converge to the diiron center and are critical for capturing substrates. The reactivity of the reduced-form Fe(II) -Fe(II) YtfE toward nitric oxide demonstrates that the prerequisite for N2 O production requires the two iron sites to be nitrosylated simultaneously. Specifically, the nitrosylation of the two iron sites prior to their reductive coupling to produce N2 O is cooperative. This result suggests that, in addition to any repair of iron centers (RIC) activity, YtfE acts as an NO-trapping scavenger to promote the NO to N2 O transformation under low NO flux, which precedes nitrosative stress.

PubMed: 27246459
DOI: 10.1002/CHEM.201600990

Experimental method

X-RAY DIFFRACTION (1.8 Å)