5FMW

The poly-C9 component of the Complement Membrane Attack Complex

5FMW の概要

• エントリーDOI: 10.2210/pdb5fmw/pdb
• EMDBエントリー: 3235
• 分子名称: POLYC9 (1 entity in total)
• 機能のキーワード: structural protein, pore-forming protein, complement, c9, macpf
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Secreted : P02748
• タンパク質・核酸の鎖数: 22
• 化学式量合計: 1266823.16

構造登録者

Dudkina, N.V.,Spicer, B.A.,Reboul, C.F.,Conroy, P.J.,Lukoyanova, N.,Elmlund, H.,Law, R.H.P.,Ekkel, S.M.,Kondos, S.C.,Goode, R.J.A.,Ramm, G.,Whisstock, J.C.,Saibil, H.R.,Dunstone, M.A. (登録日: 2015-11-10, 公開日: 2016-02-17, 最終更新日: 2024-05-08)

主引用文献

Dudkina, N.V.,Spicer, B.A.,Reboul, C.F.,Conroy, P.J.,Lukoyanova, N.,Elmlund, H.,Law, R.H.P.,Ekkel, S.M.,Kondos, S.C.,Goode, R.J.A.,Ramm, G.,Whisstock, J.C.,Saibil, H.R.,Dunstone, M.A.
Structure of the Poly-C9 Component of the Complement Membrane Attack Complex
Nat.Commun., 7:10588-, 2016

PubMed Abstract: The membrane attack complex (MAC)/perforin-like protein complement component 9 (C9) is the major component of the MAC, a multi-protein complex that forms pores in the membrane of target pathogens. In contrast to homologous proteins such as perforin and the cholesterol-dependent cytolysins (CDCs), all of which require the membrane for oligomerisation, C9 assembles directly onto the nascent MAC from solution. However, the molecular mechanism of MAC assembly remains to be understood. Here we present the 8 Å cryo-EM structure of a soluble form of the poly-C9 component of the MAC. These data reveal a 22-fold symmetrical arrangement of C9 molecules that yield an 88-strand pore-forming β-barrel. The N-terminal thrombospondin-1 (TSP1) domain forms an unexpectedly extensive part of the oligomerisation interface, thus likely facilitating solution-based assembly. These TSP1 interactions may also explain how additional C9 subunits can be recruited to the growing MAC subsequent to membrane insertion.

PubMed: 26841934
DOI: 10.1038/NCOMMS10588

実験手法

ELECTRON MICROSCOPY (6.7 Å)