5FMB

Structure of D80A-fructofuranosidase from Xanthophyllomyces dendrorhous complexed with fructose and HEPES buffer

Summary for 5FMB

• Entry DOI: 10.2210/pdb5fmb/pdb
• Descriptor: BETA-FRUCTOFURANOSIDASE, beta-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• Functional Keywords: hydrolase, carbohydrates, catalysis, catalytic domain, dimerization, quaternary, pichia pastoris, glycoside hydrolases, fungal proteins, kinetics, molecular conformation, substrate specificity, beta-fructofuranosidase, invertase, prebiotics, fructose, hepes buffer
• Biological source: Xanthophyllomyces dendrorhous (PHAFFIA RHODOZYMA)
• Total number of polymer chains: 2
• Total formula weight: 157451.82

Authors

Ramirez-Escudero, M.,Sanz-Aparicio, J. (deposition date: 2015-11-02, release date: 2016-02-10, Last modification date: 2024-01-10)

• Primary citation: Ramirez-Escudero, M.,Gimeno-Perez, M.,Gonzalez, B.,Linde, D.,Merdzo, Z.,Fernandez-Lobato, M.,Sanz-Aparicio, J.; Structural Analysis of Beta-Fructofuranosidase from Xanthophyllomyces Dendrorhous Reveals Unique Features and the Crucial Role of N-Glycosylation in Oligomerization and Activity; J.Biol.Chem., 291:6843-, 2016; PubMed: 26823463; DOI: 10.1074/JBC.M115.708495

Experimental method

X-RAY DIFFRACTION (1.91 Å)