5FL7

Structure of the F1c10 complex from Yarrowia lipolytica ATP synthase

5FL7 の概要

• エントリーDOI: 10.2210/pdb5fl7/pdb
• 分子名称: ATP SYNTHASE SUBUNIT ALPHA, ATP SYNTHASE SUBUNIT BETA, ATP SYNTHASE SUBUNIT GAMMA CHAIN, MITOCHONDRIAL, ... (10 entities in total)
• 機能のキーワード: hydrolase, atp synthase family, nucleotide binding, proton transporting, rotational mechanism, atp biosynthetic process, atp synthesis/hydrolysis
• 由来する生物種: YARROWIA LIPOLYTICA; 詳細
• 細胞内の位置: Mitochondrion membrane ; Multi- pass membrane protein : Q37695
• タンパク質・核酸の鎖数: 19
• 化学式量合計: 466474.66

構造登録者

Parey, K.,Bublitz, M.,Meier, T. (登録日: 2015-10-22, 公開日: 2016-06-29, 最終更新日: 2024-01-10)

主引用文献

Hahn, A.,Parey, K.,Bublitz, M.,Mills, D.J.,Zickermann, V.,Vonck, J.,Kuhlbrandt, W.,Meier, T.
Structure of a Complete ATP Synthase Dimer Reveals the Molecular Basis of Inner Mitochondrial Membrane Morphology.
Mol.Cell, 63:445-, 2016

PubMed Abstract: We determined the structure of a complete, dimeric F1Fo-ATP synthase from yeast Yarrowia lipolytica mitochondria by a combination of cryo-EM and X-ray crystallography. The final structure resolves 58 of the 60 dimer subunits. Horizontal helices of subunit a in Fo wrap around the c-ring rotor, and a total of six vertical helices assigned to subunits a, b, f, i, and 8 span the membrane. Subunit 8 (A6L in human) is an evolutionary derivative of the bacterial b subunit. On the lumenal membrane surface, subunit f establishes direct contact between the two monomers. Comparison with a cryo-EM map of the F1Fo monomer identifies subunits e and g at the lateral dimer interface. They do not form dimer contacts but enable dimer formation by inducing a strong membrane curvature of ∼100°. Our structure explains the structural basis of cristae formation in mitochondria, a landmark signature of eukaryotic cell morphology.

PubMed: 27373333
DOI: 10.1016/J.MOLCEL.2016.05.037

実験手法

X-RAY DIFFRACTION (3.5 Å)